CLINICAL AND HAEMATO-BIOCHEMICAL STATUS FOLLOWING CRYSTALLOID AND COLLOID FLUID THERAPY IN DYSTOCIA AFFECTED BUFFALO SUBJECTED TO OBSTETRICAL MANEUVERING

Abstract

The impact of crystalloid (Normal or Hypertonic saline) and colloid (Dextran-40 or Polygeline) based fluid
therapies on clinical and haemato-biochemical status as well as survival rate of 24 dystocia-affected buffalo
subjected to obstetrical maneuvering was assessed. Depending upon fluid therapy administered, the buffalo
were divided (n=6 each) into groups namely, group NSS (Normal saline solution 5-10 L, i.v.), group H+O (7.2%
Hypertonic saline solution, HSS @ 4 ml/kg b wt, i.v. + Oral fluid/freshwater @ 40 ml/kg b wt), group D+H+O
(Dextran-40 @ 20 ml/kg b wt, i.v. + HSS + Oral fluid) and group P+H+O (Polygeline @ 20 ml/kg b wt, i.v. +
HSS + Oral fluid). Blood samples following delivery of fetus were collected immediately before the start (0 h)
and at 6, 12 and 24 h after the start of fluid therapy. Heart rate decreased (p<0.05) in groups H+O and D+H+O.
Capillary refill time, degree of dehydration and packed cell volume decreased (P<0.05) at 24 h in groups D+H+O
and P+H+O. Rectal temperature, respiratory rate and peripheral pulse rate exhibited no variation (P>0.05). The
decline (P<0.05) in blood glucose was better in groups D+H+O and P+H+O as compared to groups NSS and
H+O. Plasma creatinine, blood urea nitrogen and plasma proteins had no variation (P>0.05) during post-fluid
therapy period. In group P+H+O, plasma sodium decreased (P<0.05) and plasma potassium increased (P<0.05)
by 24 h after fluid therapy, whereas, plasma chloride remained unchanged (p>0.05). The dam survival rate was
better in colloid compared to crystalloid-based fluid therapies (83.3-100% vs. 66.6%, respectively). In conclusion,
colloid-based fluid therapy was more effective for the resuscitation of buffalo subjected to obstetrical maneuvering.