COMPARISON OF INITIAL PARAMETERS AND FUNCTIONAL ATTRIBUTES AT DIFFERENT STAGES OF PROCESSING IN FREEZABLE AND NONFREEZABLE SEMEN

Abstract

Six Murrah buffalo breeding bulls with the history of donating semen of good freezability and fertility
parameters, and another six Murrah bulls frequently donating either initial poor quality semen or higher degree
of damage during processing were selected to study their functional parameters viz. motility, live and dead count,
reaction to hypo-osmotic solution (HOS) and acrosomal integrity at four stages viz. immediately post-dilution at
37°C, post-equilibration at 4°C and at 0 h and 1 h post-thaw. In brief, it was concluded that a higher (P<0.05-0.01)
livability, progressive motility, HOS activity and percent intact acrosomes was present in freezable ejaculates at all
the four stages of processing, in comparison to samples with poor freezability