USE OF GLYCEROL AT 8% CONCENTRATION DURING CYROPRESERVATION IS OPTIMAL FOR THE POST-THAW QUALITY OF CAUDA EPIDIDYMAL SPERMATOZOA OF RAM

Authors

  • T AHMED Division of Animal Reproduction, Gynaecology and Obstetrics S. K. University of Agricultural Sciences and Technology (K), Srinagar-190 006
  • R ISLAM

Keywords:

Cryopreservation, Glycerol, Post thaw, Ram, Spermatozoa

Abstract

The cauda epididymal spermatozoa of ram recovered using incision method and showing progressive motility
≥70% were pooled and divided into aliquots before extension in Tris-Citric acid-Fructose-Egg yolk extender
using glycerol at 4% (G4), 6% (G6) and 8% (G8) concentration as cryoprotectant. At post-thaw stage, the sperm
motility was higher (P<0.05) for G8 than G4. At pre-freeze stage, the hypoosmotic swelling test (HOST) reacted
spermatozoa were higher (P<0.05) for G8 and G6 than G4. However, at post-thaw stage, the HOST reacted
spermatozoa were higher (P<0.05) for G8 than both G6 and G4. In conclusion, 8% glycerol provides better
sperm motility and sperm membrane integrity and is recommended for the freezing of ram cauda epididymal
spermatozoa

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Published

2016-08-28

Issue

Vol. 38 No. 1 (2017): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

AHMED, T., & ISLAM, R. (2016). USE OF GLYCEROL AT 8% CONCENTRATION DURING CYROPRESERVATION IS OPTIMAL FOR THE POST-THAW QUALITY OF CAUDA EPIDIDYMAL SPERMATOZOA OF RAM. The Indian Journal of Animal Reproduction, 38(1), 23–25. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/3582