MELATONIN AS AN ADDITIVE TO SHIELD THE HAZARDOUS EFFECTS OF CRYOPRESERVATION ON KANKREJ BULL SEMEN

Authors

  • P S KAPADIYA Department of Gynaecology and Obstetrics Sardarkrushinagar Dantiwada Agricultural University, Deesa - 385 535
  • H C NAKHASHI
  • T V SUTARIA
  • B S RATHOD
  • B N SUTHAR

Keywords:

Cryopreservation, Kankrej bull,, Melatonin, Semen, Seminal characteristics

Abstract

Melatonin was supplemented at 1, 2 and 3 mM in Tris-Fructose Egg Yolk Citrate Glycerol (TFYG) extender
to assess the impact on various seminal and acrosomal characteristics of 24 ejaculates collected from Kankrej
bulls (n=3). In brief, melatonin supplementation at 2 mM increased (p<0.05) percent sperm motility, sperm
viability, HOST reactive spermatozoa, live sperm with intact acrosome and acrosomal integrity. In addition, 2 mM
melatonin supplementation reduced (p<0.05) percent dead spermatozoa, dead sperm with damaged acrosome,
dead sperm with intact acrosome and abnormal spermatozoa at post-dilution, post-equilibration and post-thaw
stages of cryopreservation

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Published

2022-12-21

How to Cite

KAPADIYA, P.S., NAKHASHI, H.C., SUTARIA, T.V., RATHOD, B.S., & SUTHAR, B.N. (2022). MELATONIN AS AN ADDITIVE TO SHIELD THE HAZARDOUS EFFECTS OF CRYOPRESERVATION ON KANKREJ BULL SEMEN. The Indian Journal of Animal Reproduction, 38(1), 16–19. Retrieved from https://acspublisher.com/journals/index.php/ijar/article/view/3559