Assessment of fungal diversity and impacts of aflatoxin B1 on physiology of maize seeds (Zea mays L.)
Keywords:
Maize seeds, mycoflora, , seed-borne, germination,, seedling growth.Abstract
Maize seeds samples were collected from three locations of Darbhanga viz. Kusheshwar Asthan, Sakri and Jale with respect to mycoflora, toxin production and maize seedling growths. The number of mycoflora Aspergillus flavus, Fusarium spp, A. niger, Penicillium spp. was recorded from all the locations maize seeds. However, among all the mycoflora, A. flavus was found to be dominant. Basically aflatoxin B1, B1B2, B1B2G2 were also produced by A. flavus in SMKY Liquid media, ranging between 0.2 and 2.7 µg/l. The impact of B1 on seed germination and seedling growth was also recorded. The maximum inhibition in seed germination were observed at 2000 ppm by variety A (75.26%) and variety B (76.66%) respectively. The maximum inhibition in root and shoot lengths was also noticed in variety A (79.49%, 68%) and Variety B (71.07%, 59.71%) at 2ppm of aflatoxin B1, respectively over all my observation conclusive that the healthy seeds is the foundation of healthy plant a necessary condition for high yields.
References
Joshi, P.K., Singh, N.P., Singh, N.P. and Vasisht, A.K. 2005. Production performance of maize crop on northern India: A district wise exploration Agricultural situation in India. LXI (11): 765-771.
Sinha, K.K., and Kumari, P.1990. Some physiological abnormalties induced by aflatoxin (AFTB) in mung seeds (Vigna radiata var. Pusa Baishakhi). Mycopathologia, 110:77-79.
Park, J.W., Kim, E.K., Kim, Y.B. 2004. Estimation of daily exposure of Koreans to aflatoxin B1 thought food consumption, Journal of Food Additives and Contaminants, 21:70-75.
J. Postharvest Technol., 2023, 11(3): 119-124 123
Rani et al. (Fungal diversity and impacts of aflatoxin B1 on physiology of maize seeds)
Koirala, P., Kumar, S., Yadar, B.K., Premarajan, K.C. 2005. Occurrence of Aflatoxin in some of the food and feed in Nepal. Indian Journal of Medicinal Science, 59: 331-336.
Domijan, A.M., Peraica, M., Zlender, V., Cvjetkovic, B., Jurjevic Z., Topolovec, P.S., Ivic, D. 2005 .Seed borne fungi in common bean seeds. Journal of Food chemical and Toxicology, 43 (3) 421-432.
Khalangwiset, P., Shephard, G.S. and W.O.F. 2011. Aflatoxins and growth impairment. Critical Reviews in Toxicology, 41 ,740- 755.
Diener, U.L., Davis, N.D. 1966. Aflatoxin production by isolation of Aspergillus flavus, Phytopathology, 56 (12): 1390-1393.
Thomas, F., Eppley, R.M., Trucksess, M.W. 1975. Rapid screening method for aflatoxins and zearalenone in corn. Journal of the Association of Official Analytical Chemists, 58 (1) :114-116.
Bathla, S., Jaidka, M., Kaur, R. 2019. Nutritive Value. In Eds Maize production and Use Intech Open. https://doi.org/10.5772/intechopen.88963.
Prasad, G. 1992. Effects of aflatoxin B1 and citrinin on physiological and biochemical processes of maize seeds, Ph.D. Thesis, T.M.B.U, Bhagalpur.
ISTA. 1999. International Rules for Seed Testing, Seed science of Technology, 23: 269.
Dospekhov, B.A. 1984. Field experimentation, statistical procedures. Mir, Moscow.
Mathur, S.B., and Kongsdal, O. 2003. Common laboratory of seed health testing methods for detecting fungi,Kandrup Bogtrykkeri , Arhusgade 88, DK-2100 Copenhagen, Den
Sulatania, D., Prasad, G., and Rani, L. 2022. Prevalence of Aflatoxin in maize grains of Madhubani District-North Bihar. Research Journal of Agricultural Science, 13(4):1311-1313.
