ESTIMATION OF POSTMORTEM INTERVAL USING RGB (RED, GREEN AND BLUE) SCORING OF CORNEAL OPACITY
Keywords:
Postmortem interval, Death, Corneal, Opacity, Digital Photography, RGB ScoringAbstract
Introduction : The Estimation of Postmortem Interval (PMI) is one of the fundamental task to be extended by all the forensic experts. The PMI is always calculated taking into consideration of all the postmortem changes in the body. The present study has taken the account corneal opacity in the estimation of PMI using digital photography and software-based scoring method for analysing the images. Material and Methods: This cross-sectional study was conducted at a tertiary health care centre in Western Maharashtra. Total of 100 cases brought to mortuary with known time since death were included in the study. The corneas of the cadavers were photographed and the images were subjected for analysis using the software in terms of RGB Scores. The entire data was analysed using SPSS ver 24.0 for MS Windows. Result : The R, G, B scores of corneal opacity shown a significant positive correlation with each other and PMI. A linear regression equation was derived using the variables for the prediction of approximate PMI.
References
Payne-James J, Busuttil A, Smock W. History and development of forensic medicine and pathology. Forensic medicine: clinical and pathological aspects London: Greenwich Medical Media. 2003.
DiMaio VJ, DiMaio D. Forensic pathology. 2 ed. Boca Ration: CRC press, LCC; 2001.
Aggrawal A. Text book of forensic medicine and toxicology. 1 ed. New Delhi: Avichal Publishing Company; 2014.
Aoki T. Studies on the estimation of time after death. Jikeikai Med J.1965;1:3-18.
Furukawa M, Funao T, Nagasaki Y. A new estimation of lapse of time since death by corneal turbidity. Kitasato Med. 1985; 15: 364-367.
Tsunenari S, Watanabe S, Takahama K, Kanda M. A method to determine the corneal turbidity by application of laser. Nihon Hoigaku Zasshi.1971; 25: 373-5.
Tsunenari S, Oho S, Sasaki S, Muto H, Kanda M. The development of the laser apparatus to determine corneal turbidity-model. Jpn J Leg Med.1974; 28: 281- 5.
Meng YZ, Fu CJ, Wang C. An experimental pathological study of the relationship between the corneal thickness and the time of death. Hubei Yikedaxue Xuebao.2001;22: 35-6.
Song G, Shi JH. Study of corneal endothelium cell and its application in forensic medicine. Forensic Sci Technol. 2001;1: 36-7.
Song GL, Shi JH, Qiao H. Estimation time of death by determining the endothelial cell living rate from corpse cornea. Chin J Forensic Med.1995;10: 90-3.
Chen X, Yi S, Liu L. Image analysis for degradation of DNA in retinal nuclei of rat after death. J Huazhong Univ Sci Technol Med Sci. 2007;27: 24-6.
Zhou L, Liu Y, Liu L, Zhuo L, Liang M, Yang F. Image analysis on corneal opacity: A novel method to estimate postmortem interval in rabbits. Journal of Huazhong University of Science and Technology [Medical Sciences]. 2010;30(2):235-9.
Liu F, Zhu S, Fu Y, Fan F, Wang T, Lu S. Image analysis of the relationship between changes of cornea and postmortem interval. Pacific Rim International Conference on Artificial Intelligence: Springer;2008.
Kawashima W, Hatake K, Kudo R, Nakanishi M, Tamaki S, Kasuda S, et al. Estimating the time after death on the basis of corneal opacity. Journal of Forensic Research. 2015;6(1):1-5.
Cantürk Ý, Çelik S, Þahin MF, Yaðmur F, Kara S, Karabiber F. Investigation of opacity development in the human eye for estimation of the postmortem interval. Biocybernetics and Biomedical Engineering. 2017;37(3):559-65.
Zhou L, Liu Y, Liu L, Zhuo L, Liang M, Yang F, et al. Image analysis on corneal opacity: A novel method to estimate postmortem interval in rabbits. Journal of Huazhong University of Science and Technology [Medical Sciences]. 2010;30(2):235-9.
Ubels J, Pruis R, Sybesma J, Casterton P. Corneal opacity, hydration and endothelial morphology in the bovine cornea opacity and permeability assay using reduced treatment times. Toxicology in vitro. 2000;14(4):379-86.
Kumar B, Kumari V, Mahto T, Sharma A, Kumar A. Determination of time elapsed since death from the status of transparency of cornea in ranchi in different weathers. J Indian Acad Forensic Med. 2012;34(4):336- 8.
