GENETIC ANALYSIS OF THE SHORT TANDEM REPEAT LOCI IN POPULATION SAMPLES FROM NAINITAL, UTTARAKHAND
Keywords:
Short Tandem Repeat, Allele Frequencies, AmpFlSTR Identifiler, Uttarakhand populationAbstract
In this study, the allele frequencies and some statistical parameters of forensic interest were determined for the fifteen short tandem repeat loci included in AmpFlSTR Identifiler (Applied Biosystems) genotyping kit in Nainital, Uttarakhand population. A total of 114 samples were analysed for the said study. All the analyzed loci met Hardy-Weinberg equilibrium expectations. They possess a high combined power of discrimination & high combined power of exclusion, showing them to be a group of excellent markers as complement in paternity testing, in the identification of individuals and in forensic applications in general.
References
1. Applied Biosystems, AmpFlSTR Identifiler PCR Amplification Kit. User’s Manual, Applera, 2001. 2. Applied Biosystems, ABI 3100 Avant Genetic Analyzer User’s Manual, Applera, 2001.
3. Power Stats V12.xls software. Free program distributed by Promega Corporation, Madison – USA over the Internet from http://w.w.w.promega.com/ techserv/apps/hmnid.
4. M.Raymond and F. Rousset. Genepop (version 1.2): population genetics software for exact tests and ecumenicism, J.Hered.1995; 86: 248-249.
5. Chattopadhyay PK, Cleef S, Duelmer M, Henke L and Henke J. Human autosomal short tandem repeat types in Jat Sikhs from North India. Forensic Sci. Int. 2000; 113: 28 – 32.
6. Dutta R and Kashyap VK. Allele frequency of six STR loci among three predominant population groups of eastern India. Forensic Sci. Int.2001; 119: 129 – 130.
7. Gaikwad S and Kashyap VK. Polymorphism at 15 hypervariable microsatellite loci in four populations of Maharashtra, India. Forensic Sci. Int.2002; 126(3): 267 – 271.
8. Gaikwad S and Kashyap VK. Genetic diversity in four tribal groups of western India: a survey of polymorphism in 15 STR loci and their application in human identification, Forensic Sci. Int.2003; 134 (2- 3): 225 – 231.
9. Kashyap VK, Guha S and Trivedi R. Concordance study on 15 STR loci in three major population of Himalayan state Sikkim. J. Forensic Sci.2000; 47: 1163 – 1167.
10. Kashyap VK, Sarkar N and Trivedi R. Allele frequencies for STR loci of the Powerplex 16 multiplex system in five endogamous populations of India. Forensic Sci. Int. 2002; 126: 180 – 188.
11. Kushwaha KPS, Rao MB and Chauhan Kamal (2007). Population genetics of 12 PCR based DNA markers among people of Haryana, India. The Ind. J. Crim. For. Sci. 28 (2): 45-51.
12. Maity B, Nunga SC and Kashyap VK. Genetic polymorphism revealed by 13 tetrameric and 2 pentameric STR loci in four Mongoloid tribal populations. Forensic Sci Int.2003; 132: 216-222.