DNA EXTRACTION FROM FORMALIN FIXED TISSUE USING AN ALTERNATIVE PROTOCOL
Keywords:
Formalin fixed tissue, DNA Extraction, de crosslinking, PCR amplification, DNA profilingAbstract
It is always a challenging task for obtaining desired amount of amplifiable DNA from formalin-fixed and non paraffin-embedded tissue sample for the purpose of human individualization, since formalin fixation of tissue samples causes fragmentation of the DNA and formation of cross linked protein-DNA complexes. The present study sought laboratories in cases related to product of conception,abandoned babies, microtomized and stained histo- pathological slides etc. needing DNA test.
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