Efficacy of sterilized culture filtrate of Trichoderma harzianum Rifai against mango wilt pathogen, Ceratocystis fimbriata Ellis and Halst
Keywords:
Mango, wilt, Ceratocystis fimbriata, Trichoderma harzianum, managementAbstract
Mango wilt disease caused by Ceratocystis fimbriata has become a serious constraint in most mango growing areas in Uttar Pradesh and in several states in India. Management of mango wilt has been achieved by the application of cultural practices and the foliar and soil application of fungicides. In this study, the sterilized culture filtrate of TH 12 was also evaluated for their efficacy against C. fimbriata through food poison technique under laboratory conditions. All the concentrations of culture filtrate of TH-12 were found effective up to 20 days but no difference in growth of control and 0.15 per cent concentration was recorded at 30th day after inoculation. The maximum suppression of C. fimbriata was found at 10 per cent concentration on 30th day after inoculation. Concentrations of filtrate used in the range from 0.15 to 10 per cent significantly reduced the growth of C. fimbriata in comparison to control. The suppressive potential of the sterilized culture filtrate of TH-12 bio-control agent indicated the possibility of development of bio formulation for the management of mango wilt under field conditions.
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Al-Adawi, A.O., Deadman, M.L., Al Rawahi, A.K., Al Maqbali, Y.M., Al Jahwari., A.A., Al Saadi, B.A., Al Amri, I.S. and Wingfield, M.J. 2006. Aetiology and causal agents of mango sudden decline disease in the Sultanate of Oman. European Journal of Plant Pathology, 116: 247-254.
Al-Yahani, R., Khan, I.A. and Deadman, A. 2005. Citrus and mango. International tropical fruits workshop. Chronic Horticulturae, 45: 42-43.
Bastos C.N. and Evans H.C. 1978. Occurrence of Ceratocystis
Doses (ml)
Radial growth of C. fimbriata (mm) 10th Day 20th Day 30th Day
fimbriata Ell. and Halst. in Brazilian Amazonia. Acta Amazonica, 8: 543-544.
6.0 9.8 14.0 5 11.6 19.0 23.8 2.5 14.2 26.8 41.0
25 22.2 32.8 51.4 0.6 26.0 44.4 54.8 0.3 28.6 45.0 56.0
15 44.0 56.4 75.4 Control 57.0 76.2 77.6 CD0.05 2.9 3.7 3.9
Deadman, M., Al-Adawi, A., Al-Yahyai, R. and Wingfield, M.J. 2007. Ceratocystis wilt of mango in Oman. Proceedings of International Symposium, Prospects of Horticulture Industry in Pakistan Publisher: Institute of Horticultural Sciences, University of Agriculture., pp. 191-194.
Dennis, C. and Webster, J. 1971a. Antagonistic properties of speciesgroups of Trichoderma I. Production of non-volatile antibiotics. Transactions of Britist Mycological Society, 57: 25- 39.
P.K. Shukla, Tahseen Fatima and Gundappa
Dennis, C. and Webster, J. 1971b. Antagonistic properties of species groups of Trichoderma II. Production of non-volatile antibiotics. Transactions of Britist Mycological Society, 57: 41- 48.
Fateh, F.S., Kazmi, M.R., Ahmad, I. and Ashraf, M. 2006. Ceratocystis fimbriata isolated from vascular bundles of declining mango trees in Sindh, Pakistan. Pakistan Journal of Botany, 38: 1257–1259.
Iton, E.F. 1960. Studies on a wilt disease of Cacao at River Estate. II. Some aspects of wind transmission. In: Annual Report on Cacao Research, 1959-1960. St Augustine, Trinidad: Imperial College of Tropical Agriculture, University of the West Indies, pp. 47-58.
Jiskani, M.M. 2002. Dying of mango orchards needs special attention. Pakistan and Gulf Economist, Karachi, March, 18– 24.
Khan, H.S.I., Ravindra, H., Ekbote, S., Narayaswamy, H., Narayanaswamy, P. and Pradeep, S. 2017. Bio efficacy of fungicides and bio agents against Ceratocystis fimbriata Ell. and Halst. causing wilt disease of pomegranate. International Journal of Current Microbiology and Applied Sciences, 6: 2902-2907.
Masood, A., Shafqat, S., Asif, S. and Mudassar, A. 2009. Life cycle and biology of mango bark beetle, Hypocryphalus mangifera Stebbing: as a possible vector of sudden death disease of mango. Pakistan Journal of Zoology, 41: 281-288.
Mishra, A., Dixit, S., Ved Ratan, Srivastava, M., Trivedi, S. and Srivastava, Y.R. 2018. Identification and in silico screening of biologically active secondary metabolites isolated from Trichoderma harzianum. Annals of Phytomedicine, 7: 78-86.
Misra, A.K. 2004. Wilt, a new serious problem in mango production in India. Proc. National Symposium on detection and management of plant diseases using conventional and modern tools, and IPS Zonal Meeting (ME Zone), December 31, 2004, CISH, Lucknow, pp. 12-13.
Misra, A.K. 2006. New emerging disease problems of some subtropical fruits in India and their management. Indian Phytopathology, 59: 375-376.
NHB Database 2017. National Horticulture Board, Department of Horticulture and Cooperation, Government of India, Aristo Printing Press, New Delhi.
Ploetz, R.C. 2003. Diseases of mango. In: Diseases of Tropical Fruits. (ed. R.C. Ploetz), CABI Publishing, U.K., pp. 327- 363.
Ploetz, R.C. and Prakash, O. 1997. Foliar, floral and soil borne diseases. In: The Mango: Botany, Production and Uses. (ed. R.E. Litz), CABI Publishing, U.K., pp. 281-326.
Reino, J. L., Guerrero, R. F., Hernandez-Galan, R. and Collado, I. G. 2008. Secondary metabolites from species of the biocontrol agent Trichoderma. Phytochemistry, 7: 89-123.
Saeed, S., Masood, A., Sajjad, A. and Zahid, D.M. 2010. Monitoring the dispersal potential of bark beetle, Hypocryphalus mangiferae Stebbing (Scolytidae: Coleoptera) in mango Orchards. Pakistan Journal of Zoology, 42: 473-479.
Shukla, P.K. 2017. Mango wilt disease management. Extension Folder, ICAR-Central Institute for Subtropical Horticulture, Rehmankhera, P.O. Kakori, Lucknow, pp. 4.
Shukla, P.K., Varma, S., Fatima, T., Mishra, R., Misra, A.K., Bajpai, A., Gundappa and Muthukumar, M. 2018. First report on wilt disease of mango caused by Ceratocystis fimbriata in Uttar Pradesh. Indian Phytopathology, 71: 135-142.
Somu, R, Mesta, R.K. and Kiran Kumar K.C. 2018. Effect of non systemic, systemic fungicides and bio control agents against wilt of pomegranate caused by Ceratocystis fimbriata. International Journal of Chemical Studies, 6: 397-402.
Statista – The portal for statistics 2016-17. https:// www.statista.com/
Van Wyk, M., Pegg, G., Lawson, S. and Wingfield, M.J. 2007. Ceratocystis atrox sp. nov associated with Phoracanthta acanthocera infestations on Eucalyptus in Australia. Australasian Plant Pathology, 36: 407-414.
