High-performance Thin-layer Chromatography Fingerprinting Analysis of Punarnavadi Kwatha

Authors

  • Resmi Vijayan PG Scholar, Department of Dravyaguna Vijnanam, GAVC, Tripunithura, Kerala, India. Author
  • P Y Ansary HOD and Professor, Department of Dravyaguna Vijnanam, GAVC, Tripunithura, Kerala, India. Author
  • Sara Moncy Oommen Professor and HOD, Department of Dravyaguna Vijnana, Government Ayurveda College, Kannur, Kerala, India. Author
  • V V Shincymol Associate Professor, Department of Dravyaguna Vijnanam, GAVC, Tripunithura, Kerala, India. Author

Keywords:

High-performance thin-layer chromatography fingerprinting Punarnavadi kwatha, Peaks, Phytoconstituents

Abstract

Introduction: Punarnavadi kwatha is one among the commonly used medicine in Ayurvedic practice, mentioned in  Chakradatta in sopha chikitsa. Main indications of Punarnavadi kwatha are Sarvanga sopha (generalised oedema),  Udara (ascites), Kasa (cough), Soolam (colicky pain), and Swasa (dyspnoea) associated with Pandu (anaemia).  For the purpose of quality assurance and uniformity, scientific verification of Ayurvedic medications is important.  Usage of modern analytical techniques helps in the proper authentication of medicines. In the present study, high performance thin-layer chromatography (HPTLC) fingerprinting of Punarnavadi kwatha aimed to assess the  different phytoconstituents. Materials and Methods: Methanolic extract of Punarnavadi kwatha was subjected to HPTLC fingerprinting using  the solvent system toluene:ethyl acetate:formic acid (6:3:0.5). Toluene is a least polar solvent, ethyl acetate is mid  polar solvent and formic acid is highly polar so in this solvent system, maximum separation of compounds took place. Results and Discussion: The number of peaks obtained at visualization 254 nm was 8 and total area of compounds  in is 30309.5. Visualization at 366 nm, number of peaks was 12 and total area is 42473.2. Conclusion: Thus, the study revealed that HPTLC fingerprinting of formulations can be used in the quality control  of ayurvedic medicaments. The maximum separation of compounds occurred at this solvent system.

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References

Sen S, editor. Chakrapani. Chakradatta. Trans-Pandita Kulapathy VA Upadhikaadikari. Sotha Chikitsa, Sloga no. 11. Varanasi:

Himasagarachandra Murthy P. Sarngadhara. Sarngadhara Samhita. Madhyama khandam. Ch. 2. Kwathakalpana Adhyaya Sloka 76-77. Varanasi: Choukhambha Sanskrit Series Office; 2013. p. 124.

Krishnan Vaidyan KV, Gopala Pillai S, editors. Sahasrayogam Vidyarambham. Udara Chikitsa. Alappuzha: Vidyarambham Publishers; 2013. p. 279.

Ministry of Health and Family Welfare. The Ayurvedic Formulary of India. 1st ed. Part 1, A. New Delhi: Government of India; 2016. p. 182-3. 6. Abraham A, Samuel S, Mathew L. Phytochemical analysis of Pathyashadangam kwath and its standardization by HPLC and HPTLC. J Ayurveda Integr Med 2020;11:153-8.

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Published

2023-12-30

How to Cite

Vijayan, R., Ansary, P.Y., Oommen, S.M., & Shincymol, V.V. (2023). High-performance Thin-layer Chromatography Fingerprinting Analysis of Punarnavadi Kwatha. International Research Journal of Ayurveda and Yoga, 6(11), 25–29. Retrieved from https://acspublisher.com/journals/index.php/irjay/article/view/12772