HPTLC Fingerprinting Profile of Vacha (Acorus calamus Linn.)
Keywords:
Acorus calamus Linn, Beta-asarone, HPTLC, Sweet flag, VachaAbstract
Introduction: Vacha (Acorus calamus Linn.) commonly called Sweet flag is a significant herb with well-recognized therapeutic potential. Choorna (powder) of its rhizome is the commonly used dosage form according to Ayurveda. High-performance thin-layer chromatography (HPTLC) fingerprinting of rhizome powder of Vacha was carried out to estimate the active constituents in the dosage form choorna. ß-asarone, which is the major phytoconstituent of rhizome, was used as standard. Materials and Methods: HPTLC plate 5 × 10 cm, pre-coated with silica gel 60 F254 TLC plates, was used as stationary phase. Toluene: ethyl acetate: acetic acid (90:10:2) was used as mobile phase. Visualization was done under ultraviolet light 254 nn and 366 nm. Results and Discussion: HPTLC chromatogram showed the presence of 3 comparable peaks at 254 nm and 2 comparable peaks at 366 nm, in methanolic extract of rhizome powder and standard. Conclusion: This approach creates profile analysis of the plant rhizome and can be helpful for quality control and further researches.
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References
Kolammal M, Aiyer Narayanan K. Pharmacognosy of Ayurvedic Drugs of Travancore-cochin. Series 1, Number 3. Thiruvananthapuram: Department of Pharmacognosy, University of Travancore; 1992. p. 43-9.
Ministry of Health and Family Welfare. The Ayurvedic Pharmacopoeia of India. 1st ed., Vol. 2., Part 1. New Delhi: Government of India; 1999. p. 168-70.
Murthy KR. Vagbhata. Ashtanga Hridaya., Translator. 6th ed., Vol. 3., Ch. 1. Uttara Sthana. Balopacaraneeya, Sloka 7-9. Varanasi: Chaukhambha Krishnadas Academy; 2012. p. 4.
Murthy KR. Vagbhata. Ashtanga Hridaya. Translator. 12th ed., Vol. 3., Ch. 39. Uttara Sthana, Rasayanavidhi, Sloka 50-53. Varanasi: Chaukhambha Krishnadas Academy; 2021. p. 389.
Sharma RK. Agnivesa. Caraka Samhita. Reprint ed. Vol. 3., Ch. 10. Cikitsa Sthana. Apasmara Cikitsa, sloka 27. Varanasi: Chaukambha Sanskrit Series Office; 2015. p. 447.
Sharma RK. Agnivesa. Caraka Samhita. Reprint ed. Vol. 3., Ch. 1. Cikitsa Sthana, Rasayana Adhyaya-Abhayamalakeeyam Rasayanapadham, Sloka 41-61. Varanasi: Chaukambha Sanskrit Series Office; 2015. p. 16-9.
Murthy KR. Vagbhata. Ashtanga Hridaya. Vol. 3., Ch. 1. Uttara Sthana, Balopacaraneeya, Sloka 45-47. Varanasi: Chaukhambha Krishnadas Academy; 2012. p. 11.
Sharma PS. Mahendra Bhogika. Dhanwantari Nighantu. editor. Varanasi: Chaukambha Publications; 2008. p. 71.
Tripathi N. Saligrama. Saligrama Nighantu Bhooshanam. Bombay: Khemaraj Srikrishnadas Prakashan; 2006. p. 112-3.
Sharma PV. Priya Nighantu. Varanasi: Chaukambha Surbharati Prakashan; 2004. p. 83.
Sharma PV. Kaiyyadeva. Kaiyyadeva Nighantu. Varanasi: Chaukambha Orientalia; 2013. p. 224.
Roy SP. Role of vacha as neurological and metabolic disorders: Evidence from ethnopharmacology, phytochemistry, pharmacology and preclinical study. J Drug Deliv Ther 2022;12:106-13.
Esfandiari E. The effects of Acorus calamus L. in preventing memory loss, anxiety, and oxidative stress on lipopolysaccharide-induced neuroinflammation rat models. Int J Prev Med 2018;9:85.
Venkatesan K. Anti-amnesic and anti-cholinesterase activities of α-asarone against scopolamine-induced memory impairments in rats. Eur Rev Med Pharmacol Sci 2022;26:6344-50.
Dhiman KS, editor. Comprehensive Technical Dossier(CTD) of Medicinal Plant used in Ayurveda. 1st ed. New Delhi: Central Council for Research in Ayurvedic Sciences; 2015. p. 5-31.
Ghosh S, Sharma AK, Kumar S, Shankar Tiwari S, Rastogi S, Srivastava S, et al. In vitro and in vivo efficacy of Acorus calamus extract against Rhipicephalus (Boophilus) microplus. Parasitol Res 2011;108:361-70.
