Detection Of Group B Rotavirus In Buffalo Calves In Haryana State Of Northern India

Authors

  • Hari Mohan Department of Animal Biotechnology, College of Veterinary and Animal Sciences, LLRUVAS, Hisar (Haryana), 125004.
  • Minakshi Department of Animal Biotechnology, College of Veterinary and Animal Sciences, LLRUVAS, Hisar (Haryana), 125004.
  • Pawan Kumar Department of Animal Biotechnology, College of Veterinary and Animal Sciences, LLRUVAS, Hisar (Haryana), 125004.
  • G Prasad Department of Animal Biotechnology, College of Veterinary and Animal Sciences, LLRUVAS, Hisar (Haryana), 125004.

Keywords:

Electropherotypes, Group A rotavirus, Group B rotavirus, RNA-PAGE, RT-PCR

Abstract

Group B rotaviruses have been found associated with sporadic cases of diarrhea in calves and adult cows. One hundred fifty six faecal samples were collected from diarrheic buffalo calves under six months of age and tested for group B rotaviruses by polyacrylamide gel electrophoresis (PAGE) and reverse transcription (RT)-PCR by targeting 260 & 961 bp of VP3 and VP4 genes, respectively). By RNA-PAGE, seven calf samples were group B rotavirus positive (long electropherotype) and all the samples were found positive by RT-PCR. To the best of our knowledge, this is the first report on the presence of buffalo group B rotavirus in Haryana state of Northern India. 

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Published

2011-12-10

Issue

Vol. 7 No. 3 (2012): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan-Mar) 2012

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Mohan, H., Minakshi, Kumar, P., & Prasad , .G. (2011). Detection Of Group B Rotavirus In Buffalo Calves In Haryana State Of Northern India . Indian Journal of Veterinary Sciences and Biotechnology, 7(3), 71–74. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/3281