Rapid Detection of Pasteurella multocida Infection by Direct Blood and Tissue Polymerase Chain Reaction in Animals and Poultry

Abstract

The aim of the present study was rapid detection of Pasteurella multocida infection by direct blood and tissue PCR technique. A total  151 samples comprising of 112 blood samples, 37 tissues samples, one nasal swab, and one bone marrow from various ruminants and  poultry were processed by cultural methods and confirmed by PM-PCR by amplifying a product of 460bp. Among them four Pasteurella  multocida were obtained from tissue samples of buffaloes and two from poultry and, one from blood sample of cow. The clinical  samples were processed for direct blood PCR (n=112 blood, 37 tissue) and direct tissue PCR (n=151) methods. Out of 112 blood samples  processed, one blood sample of cow positive for P. multocida by cultural method was also found to be positive by direct blood PCR. Out  of 151 samples processed for direct tissue PCR, six tissue samples positive for P. multocida by cultural methods were also found to be  positive by direct tissue PCR. Conventional cultural method as well as PM-PCR for identification of P. multocida infection in animals and  poultry requires minimum 24-48 hrs for identification, while direct blood and tissue PCR gives specific diagnosis within 3 hrs once the  sample is received at laboratory. Thus direct application of PCR on blood and tissues will enable to give rapid diagnosis of P. multocida  infection during the outbreaks in animals and poultry.