Comparative Nucleotide Sequence Analysis of Glycoprotein B, C, and G of Infectious Laryngotracheitis Virus Isolated in Egypt During 2016-2018

Abstract

Infectious Laryngotracheitis (ILT) is an economically important disease of poultry, causing a high mortality rate and /or reduced egg
production. Tracheas from sever morbid and recently died unvaccinated household chicken were subjected to PCR assay to detect
the presence of the virus through amplification of the glycoprotein B gene. All tested samples gave positive ct
ranging from 13.32 to
25.53. Virus isolation was performed by inoculation—the processed tracheal swaps onto the chorioallantoic membrane. Positive pock
lesion has been developed within 7 days post-inoculation. The pock lesions were subjected to PCR assay for amplification of both the
full-length glycoprotein B, C, and G for sequencing analysis. Positive amplicons migrating about 2600, 1250, and 890 bp were amplified
corresponding to the orf of gpB, gpC and gpG genes. Sequence alignment and phylogenetic tree revealed that all sequenced isolates
gave a high degree with wild type isolate of ILT and a high degree of genetic stability was clearly evident among strains isolated in
different periods (2016-2018), indicating that these glycoproteins could be used as a vaccine candidate.