Prevalence of Antimicrobial Resistance in Escherichia coli isolated from Dairy Supply Chain by Phenotypic Methods

Authors

  • Amarjeet Kumar National Referral Center, ICAR-National Dairy Research Institute, Karnal-132001. Haryana, India.
  • Raghu Hirikyathanahalli Vishweswaraiah National Referral Center, ICAR-National Dairy Research Institute, Karnal-132001. Haryana, India.
  • Rashmi Hogarehalli Mallappa Molecular Biology Unit, Dairy Microbiology Division, ICAR National Dairy Research Institute, Karnal-132001. Haryana, India.
  • Gunaseka Bharath Molecular Biology Unit, Dairy Microbiology Division, ICAR National Dairy Research Institute, Karnal-132001. Haryana, India.
  • Brijesh Kumar National Referral Center, ICAR-National Dairy Research Institute, Karnal-132001. Haryana, India.
  • Avinash Jaswal National Referral Center, ICAR-National Dairy Research Institute, Karnal-132001. Haryana, India.
  • Naresh Kumar National Referral Center, ICAR-National Dairy Research Institute, Karnal-132001. Haryana, India.

Keywords:

Antimicrobial resistance, E. coli, ESBL, Milk, Multidrug-resistant

Abstract

A total of 139 E. coli positive isolates from 122 raw milk (57.2%), 10 pasteurized milk (33.3%), and 7 human handlers (35%) were identified  by phenotypic methods and 22 of the isolates were further confirmed by PCR. All 22 E. coli (100%) isolates were found resistant to  penicillin, oxacillin, erythromycin, and clindamycin. Amongst E. coli isolates, antibiotics resistance was detected towards cefotaxime  and amoxyclave (18.7% each) followed by ampicillin (7.98%), trimethoprim (15.82%), tetracycline (10.79%), nalidixic acid (7.91%), and  piperacillin (7.79%). Out of 139 isolates, 7.91% isolates were found to be MDR (multidrug resistant) and 4 isolates were confirmed as  extended spectrum β-lactamase (ESBL) producers. 

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Published

2020-12-25

Issue

Vol. 16 No. 2,3,4 (2020): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Oct-dec) 2020

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kumar, A., Vishweswaraiah, R.H., Mallappa, R.H., Bharath, G., Kumar, B., Jaswal, A., & Kumar, .N. (2020). Prevalence of Antimicrobial Resistance in Escherichia coli isolated from Dairy Supply Chain by Phenotypic Methods. Indian Journal of Veterinary Sciences and Biotechnology, 16(2,3,4), 12–16. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2387