Molecular Detection and Sequencing of Peste Des Petits Ruminants Virus of Saurashtra Region of Gujarat, India

Authors

  • Mahesh M. Tajpara Krishi Vigyan Kendra, Junagadh Agricultural University, Junagadh, India,
  • Nalinkant M. Shah Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
  • Bhaveshkumar B. Javia Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
  • Dilipsinh B. Barad Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India
  • Umed V. Ramani Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, India
  • Dharmesh R. Patel Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Navsari Agricultural University, Navsari, India
  • Vinay A. Kalaria Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh, India

Keywords:

Molecular detection, Peste Des Petits Ruminants virus, (PPR virus), Sequencing, Saurashtra region, Gujarat

Abstract

The present study was undertaken with the objective of molecular detection and sequencing of the Saurashtra region’s Peste Des Petits  Ruminants virus. A total of 119 clinical samples (40 nasal swabs, 20 conjunctiva swabs, 16 oral swabs, and 19 tissues from goats along  with 22 nasal swabs and 2 tissues of sheep lung and intestine) were tested by N gene & F gene-based primers. Out of 119 samples,  35 samples were positive for N gene primer, and 32 positives for F gene primer. The N gene-based sequence analysis of three PPRV  obtained from Bhavnagar, Amreli, and Rajkot belonged to lineage IV (Indian strains), and all three PPRV sequences showed 100 %  genetic identity with each other and 98-99 % genetic identity with PPRV strain of Mehsana (North Gujarat), Bhopal (Madhya Pradesh),  Pune (Maharashtra), Jhansi (Uttar Pradesh), and Sungri (vaccine strain)  

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Published

2021-10-10

Issue

Vol. 17 No. 4 (2021): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Sept- Oct) 2021

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Tajpara, M.M., Shah, N.M., Javia, .B.B., Barad, D.B., Ramani, U.V., Patel, D.R., & Kalaria, V.A. (2021). Molecular Detection and Sequencing of Peste Des Petits Ruminants Virus of Saurashtra Region of Gujarat, India. Indian Journal of Veterinary Sciences and Biotechnology, 17(4), 17–22. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2321