Phylogenetic Analysis and Antimicrobial Resistance of Escherichia coli Isolated from Diarrheic Piglets

Authors

  • Uma M. Tumlam Department of Veterinary Microbiology, Krantisinh Nana Patil College of Veterinary Science, Shirwal Dist. Satara, India
  • Mrunalini M. Pawade Department of Veterinary Microbiology, Krantisinh Nana Patil College of Veterinary Science, Shirwal Dist. Satara, India
  • Dushyant M. Muglikar Department of Veterinary Microbiology, Krantisinh Nana Patil College of Veterinary Science, Shirwal Dist. Satara, India
  • Dhruv N. Desai Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Navsari, India
  • Bhupesh P. Kamdi 3 Department of Veterinary Pathology, Krantisinh Nana Patil College of Veterinary Science, Shirwal, Dist. Satara, India

Keywords:

: Antibiotic drug resistance, Phylogenetic analysis, Piglets Diarrhea

Abstract

Escherichia coli is one of the major causative agents of diarrhea in neonatal piglets worldwide. The present study was conducted to
examine the occurrence of E. coli carrying virulence genes and antibiotic resistance associated with piglet diarrhea in and around Shirwal,
District Satara. 30 fecal samples were randomly collected from the piglets with history of diarrhea for the isolation of E. coli followed by
screening of virulence genes among the isolates by specific PCR. Further E. coli isolates were subjected to study phenotypic antibiotic
resistance by disc diffusion method. The overall occurrence of E. coli was 40% (12/30). On molecular characterization, all the E. coli
isolates were found positive for 16S ribosomal RNA gene. The highest antibiotic resistance was found for Amikacin (100%) followed by
Cloxacillin/Clavulate, Chloramphenicol, Enrofloxacin, Gentamicin and Metronidazole.

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Published

2022-07-10

Issue

Vol. 18 No. 3 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (July- Aug) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Tumlam, U.M., Pawade, .M.M., Muglikar, D.M., Desai, D.N., & Kamdi, B.P. (2022). Phylogenetic Analysis and Antimicrobial Resistance of Escherichia coli Isolated from Diarrheic Piglets . Indian Journal of Veterinary Sciences and Biotechnology, 18(3), 119–121. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2206