Molecular Characterization of L. monocytogenes Isolated From Milk and Milk Products

Authors

  • Shubhangi Rambhau Warke Department of Veterinary Microbiology and Animal Biotechnology T & R cell, Nagpur Veterinary College, Maharashtra Animal &Fishery Sciences University, Seminary Hills, Nagpur -440006, India.

Keywords:

: Antibiotic sensitivity tes, , L. monocytogenes, Milk & Milk products, PCR

Abstract

A total of 194 (121 bovine milk samples and 73 milk products) were screened for Listeria monocytogenes. The isolation was attempted by
two-step enrichment procedure in UVM-1 and UVM- 2 broths followed by plating on DRIA. The isolates were confirmed by biochemical
test and in-vitro PI-PLC pathogenicity test. The L. monocytogenes isolates from milk and milk products were also tested for virulenceassociated genes plcA, hlyA, actA, and iap by multiplex polymerase chain reaction (PCR). Prevalence of L. monocytogenes found to be 15.70% and 10.95% in milk and milk products, respectively. The PCR profile of L. monocytogenes isolates revealed variation in the presence of the virulence-associated genes namely plcA, hlyA, actA, and iap. Four L. monocytogene isolates from milk samples and two isolates from milk products revealed PI-PLC activity. L. monocytogenes isolates were sensitive to ampicillin, penicillin, ciprofloxacin and vancomycin

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Published

2022-08-14

Issue

Vol. 18 No. 3 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (July- Aug) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Warke, S.R. (2022). Molecular Characterization of L. monocytogenes Isolated From Milk and Milk Products. Indian Journal of Veterinary Sciences and Biotechnology, 18(3), 108–111. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2203