Prevalence of Aflatoxin M1 in Milk of Bovine in and around Junagadh, Gujarat State of India

Authors

  • Jay R. Hedpara Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Junagadh-362 001, Kamdhenu University, India
  • Samiulla H. Sindhi Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Junagadh-362 001, Kamdhenu University, India
  • Sanjay N. Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Junagadh-362 001, Kamdhenu University, India
  • Binod Kumar Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Junagadh-362 001, Kamdhenu University, India
  • Kirit R. Bhedi Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Junagadh-362 001, Kamdhenu University, India

Keywords:

: Aflatoxin M1 (AFM1, ELISA, Lateral Flow Assay, Milk

Abstract

Aflatoxin M1 (AFM1) is a hydroxylated metabolite of aflatoxin B1 from a contaminated feed. Aflatoxins are toxic, mutagenic and
carcinogenic compounds produced as a secondary metabolite by the fungi Aspergillus flavus and A. parasiticus. The present study was
conducted to determine the prevalence of AFM1 in milk by using two different diagnostic methods, viz., Enzyme-Linked Immunosorbent
Assay (ELISA) and Lateral Flow Assay (LFA). A total of 200 milk samples were collected from the areas under Junagadh city and nearby
villages. An overall 93.5% incidence rate of AFM1 contamination in milk samples was recorded. Out of 200 samples, 6 (3%) samples
were found to contain AFM1 above the FSSAI standard limit (0.5 µg/L) and 145 (72.5%) samples had AFM1 above the standard limit as
per EU legislation (0.05 µg/L). Out of total 200 samples, 50 representative samples ana

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Published

2022-07-10

How to Cite

Hedpara, J.R., Sindhi, S.H., N., .S., Kumar, .B., & Bhedi, K.R. (2022). Prevalence of Aflatoxin M1 in Milk of Bovine in and around Junagadh, Gujarat State of India. Indian Journal of Veterinary Sciences and Biotechnology, 18(3), 104–107. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2202