Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Assam, India

Authors

  • Pubaleem Deka Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
  • Mrinal K. Nath Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India
  • Sangeeta Das Department of Veterinary Microbiology, LUVAS, Hisar, Haryana, India
  • Pankaj Deka Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Asam, India
  • Ritam Hazarika Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assdam, India
  • Gunajit Das Livestock Research Station, Assam Agricultural University, Mandira, India
  • Mihir Sarma Livestock Research Station, Assam Agricultural University, Mandira, India

Keywords:

: Newcastle disease virus, Poultry, primers, RT-PCR

Abstract

A study was carried out to detect the Newcastle disease virus (NDV) from various field outbreaks in Assam. Nine hundred ninety-two
clinical and post -mortem samples during 2018-2021 were collected from the backyard and commercial poultry farms and subjected
to molecular diagnosis of NDV by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). NDV could be detected in 58.78% tissue
samples and 22.55% cloacal swabs collected from suspected poultry cases by RT-PCR. Organ wise study suggests that highest NDV
(92.94%) was detected in trachea followed by spleen (90.62%), lung (86.95%), caecal tonsils (77.08%), proventriculus (60.81%), brain
(48.91%), intestines (31.50%) and kidney (23.07%). Hence, the collection of specific tissue samples from field outbreaks for the detection of NDV is of paramount importance. In addition, RT-PCR can be considered a highly sensitive detection method for rapid and confirmative diagnosis of Newcastle disease virus.

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Published

2022-07-10

Issue

Vol. 18 No. 3 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (July- Aug) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Deka, P., Nath, M.K., Das, S., Deka, .P., Hazarika, R., Das, .G., & Sarma, M. (2022). Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Assam, India. Indian Journal of Veterinary Sciences and Biotechnology, 18(3), 97–99. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2200