Genetic variations in Toll-like receptor-4 and Myeloid differentiation protein-2 Genes among Giriraja and Indigenous Chicken

Authors

  • Sarathi N. Ramesh Department of Animal Genetics and Breeding, Veterinary College, Hebbal, Bengaluru - 560 024
  • Nagaraja Ramakrishnappa Veterinary College, Gadag – 582 101, India.
  • Naveen K. Siddaraju Department of Animal Genetics and Breeding, Veterinary College, Hebbal, Bengaluru - 560024
  • Yathish H. Maheshwarappa Department of Animal Genetics and Breeding, Veterinary College, Hebbal, Bengaluru - 560 024
  • Nagaraja C. Sringeshwaraiah Veterinary College, Hebbal, Bengaluru – 560 024, India.

Keywords:

Disease resistance, Genetic polymorphism, MD-2, PCR-RFLP, TLR4

Abstract

The present study aimed to identify genetic polymorphism in chicken Toll-like receptor-4 (TLR4) and Myeloid differentiation protein-2  (MD-2) genes in Indigenous and Giriraja birds. Blood samples were collected from 50 each of indigenous and Giriraja birds and genomic  DNA was isolated. Published primers were used to amplify chicken TLR4 (exon 3) and MD-2 (exon 1) genes. The polymorphism was  determined in TLR4 and MD-2 genes using RFLP analysis employing TaqI and AseI restriction enzymes, respectively. RFLP analysis of TLR4 gene revealed a monomorphic pattern both in Indigenous and Giriraja birds. Whereas RFLP analysis of MD-2 gene in both Indigenous  and Giriraja birds showed polymorphic patterns. In indigenous birds, genotypic frequencies were 0.02, 0.14, and 0.84 for AA, AB, and  BB, respectively, and gene frequencies were 0.09 and 0.91 for alleles A and B. In Giriraja birds, genotypic frequencies were 0.02, 0.46,  and 0.52 for AA, AB, and BB, respectively, and gene frequencies were 0.25 and 0.75 for alleles A and B. Sequence Alignment of A and B  alleles of MD-2 gene showed a SNP (G → A) at nucleotide position 73 bp in B allele. 

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Published

2022-09-19

Issue

Vol. 18 No. 2 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Apr- Jun) 2022

Section

Articles

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How to Cite

Ramesh, S.N., Ramakrishnappa, N., Siddaraju, N.K., Maheshwarappa, .Y.H., & Sringeshwaraiah, N.C. (2022). Genetic variations in Toll-like receptor-4 and Myeloid differentiation protein-2 Genes among Giriraja and Indigenous Chicken . Indian Journal of Veterinary Sciences and Biotechnology, 18(2), 26–31. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2150