Study on ABCG2 Gene Polymorphism in Jaffarabadi and Surti Buffaloes by PCR-RFLP

Authors

  • Santul Patel Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
  • Balkrishna Brahmkshtri Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
  • Umed Ramani Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
  • Guarav Pandya Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
  • Nikhil Dangar Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
  • Savita Devkatte Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India
  • Vishnu Kharadi Department of Animal Biotechnology, College of Veterinary Science & Animal Husbandry, Kamdhenu University, Navsari, Gujarat, India

Keywords:

ABCG2 gene, Jaffarabadi, Surti buffaloes, Milk production and composition trait, PCR-RFLP, Polymorphism

Abstract

In the present study, considering the role of ABCG2 gene in milk production and composition traits, Jaffarabadi and Surti buffaloes were
screened for its polymorphism. PCR- RFLP technique was used to detect the polymorphism in 292 bp (partial intron 13 and exon 14) and
240 bp (partial exon 14) fragments of ABCG2 gene using HhaI and PstI restriction enzymes, respectively. The absence of polymorphism
in ABCG2 gene in the study for partial intron 13 and exon 14 fragment of 292 bp as well as partial exon 14 fragment of 240 bp indicated
fixation of alleles at both the loci in Jaffarabadi and Surti buffalo breeds.

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Published

2022-07-11

Issue

Vol. 18 No. 3 (2022): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (July- Aug) 2022

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Patel, S., Brahmkshtri, .B., Ramani, U., Pandya, G., Dangar, N., Devkatte, .S., & Kharadi, V. (2022). Study on ABCG2 Gene Polymorphism in Jaffarabadi and Surti Buffaloes by PCR-RFLP. Indian Journal of Veterinary Sciences and Biotechnology, 18(3), 26–29. Retrieved from https://acspublisher.com/journals/index.php/ijvsbt/article/view/2140