Effect of Gonadotrophin Releasing Hormone (GnRH) on Induction of Ovarian Cyclicity in Pubertal Anoestrus Buffalo Heifers

Abstract

Effect of GnRH on induction of ovarian cyclicity was studied on 21 pubertal anoestrus buffalo heifers. Animals were divided into three 
groups. In the experimental group treated with 1.5 ml GnRH, 85.71% heifers exhibited oestrus within 83.02± 10.04 (66-90) hrs after the GnRH injection. In the second experimental group treated with 2.5 ml GnRH 71.42% heifers 
exhibited oestrus· within 62.30± 17.22 ( 42-90) hrs after the GnRH injection whereas in the control 
group 28.5% heifers exhibited oestrus within 90.00±00 (90-90) hrs after normal saline injection. Tfle pregnancy rate in the treated group 
were 4 / 5 (80%) and 4 / 5 (80%) respectively as compared to 2 / 2 ( 100%). The results indicated thatthe ovarian cyclicity can be induced 
with GnRH in anoestrus pubertal buffalo heifers.