Dr 16S rRNA Sequence Indicates Presence of Unculturable  Bacteria in Postpartum Uterus of Buffalo

Authors

  • Shabaz Hussain Dar Division of Animal Reproduction,zatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Salauddin Qureshi Division of Bacteriology and Mycology,,Izatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Muthu Sankar Division of Bacteriology and Mycology Indian Council of Agricultural Research (ICAR)-Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Sanjeev Mehrotra Division of Animal Reproduction, Izatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Manas K Patra Division of Animal Reproduction, Izatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Sanjay K Singh Division of Animal Reproduction, Izatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Harendra Kumar Division of Animal Reproduction, Izatnagar, Bareilly 243 122 Uttar Pradesh, India
  • Narayanan Krishnaswamy Division of Animal Reproduction, Izatnagar, Bareilly 243 122 Uttar Pradesh, India

DOI:

https://doi.org/10.48165/ijar.2023.44.02.17

Keywords:

She buffalo, postpartum period, Bacteria, Endometritis, PCR

Abstract

Bacterial contamination of the uterus is inevitable during the first three weeks of calving in the cow. The potential  importance of unculturable bacteria in the establishment of postpartum endometritis is reported in the cow; however,  existence of unculturable bacteria is not known in the postpartum uterus of the buffalo. Accordingly, sterile endometrial  swab was collected in peptone water on day 20-21 post-calving from Murrah buffalo (n=12) without vaginal contamination  and bacterial gDNA was isolated. Using gene specific primers, 16S rRNA was amplified by PCR. The purified PCR  product was sequenced and was compared in Ribosomal database project (RDBP). Genetic distance was calculated using  multiple alignments with ClustalW model in MegAlign module of Lasergene Package. The phylogenetic relationship was  analyzed using the software MEGA 5.1 by neighbourhood joining method. The results revealed that invariably every  sample contained uncharacterized bacteria (100%); however, no new species of was found. The clinical significance of  uncharacterized bacteria in the establishment of postpartum endometritis needs to be determined in the buffalo. 

 

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Published

2023-12-29

Issue

Vol. 44 No. 2 (2023): The Indian Journal of Animal Reproduction (Dec) 2023

Section

Short Communication

How to Cite

Dar, S.H., Qureshi, S., Sankar, M., Mehrotra, S., Patra, M.K., Singh, S.K., … Krishnaswamy, N. (2023). Dr 16S rRNA Sequence Indicates Presence of Unculturable  Bacteria in Postpartum Uterus of Buffalo. The Indian Journal of Animal Reproduction, 44(2), 90–92. https://doi.org/10.48165/ijar.2023.44.02.17
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