Comparative Efficacy of Three Different Fixed Timed Artificial Insemination Protocols in Sahiwal Cows

Authors

  • A. Singh Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab
  • N. Singh Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab
  • P. S. Brar Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab
  • V. S. Malik Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab
  • G. Singla Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab

DOI:

https://doi.org/10.48165/ijar.2021.42.2.11

Keywords:

CIDR, Fixed Timed AI, Ovsynch, Ovulation, Pregnancy, Sahiwal

Abstract

The study evaluated efficacy of three different fixed timed estrus synchronization  protocols administered at random stage of estrous cycle in Sahiwal cows.  Pluriparous Sahiwal cows (n=30) having normal estrous cycle and genitalia were  randomly divided into three groups. Group 1 (Ovsynch group), cows (n=10)  were administered standard Ovsynch protocol, Group 2 (Ovsynch + CIDR); cows  (n=10) were administered Ovsynch protocol along with intravaginal insertion  of CIDR implant on day 0, Group 3 (Estradiol based group); cows (n=10) were  injected Estradiol benzoate 2 mg + Cloprostenol 500 µg along with insertion of  CIDR on day 0, then Estradiol benzoate 1 mg + Cloprostenol 500 µg + eCG 400 IU  (Inj. Folligon 400 IU) on Day 7. CIDR was removed at the time of prostaglandin  injection (Day 7) in the group 2 and 3. All the cows were fixed timed inseminated  on Day 10 of the protocol. Results revealed that the growth rate of dominant  follicle from day 7 to 10 was higher in Estradiol based and Ovsynch + CIDR group  than Ovsynch group (1.42±0.14, 1.38±0.13 and 1.13±0.07 mm, respectively).  Average size of dominant follicle on day 10 was higher in Ovsynch + CIDR than  the Ovsynch and Estradiol based group (11.4±0.48 vs 10.3±0.61 vs 10.2±0.42 mm,  respectively). All cows of Ovsynch+ CIDR group underwent ovulation, whereas,  60 percent cows ovulated in the group 1 and 2. The ovulating cows had higher  average diameter of preovulatory follicle on day 10 compared to anovulatory cows  in the Ovsynch (11.6±0.20 vs 8.4± 0.80 mm, p<0.05) and Estradiol based group  (10.5±0.56 vs 9.9±0.66 mm, p>0.05). Higher pregnancy rate was achieved in the  Ovsynch + CIDR group compared to Estradiol based and the Ovsynch group (60  vs 40 and 30 percent, respectively). Results showed that Ovsynch + CIDR protocol  was more effective on random day of estrous cycle in inducing ovulatory estrus  and subsequent fertility compared to the Ovsynch and Estradiol based protocol. It  was also concluded that higher diameter of dominant follicle on day 10 and follicle  growth rate from day 7-10 favored ovulation and establishment of pregnancy in  Sahiwal cows.  

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Published

2021-11-11

Issue

Vol. 42 No. 2 (2021): Indian Journal of Animal Reproduction

Section

Research Article

How to Cite

Singh, A., Singh, N., Brar, P.S., Malik, V.S., & Singla , G. (2021). Comparative Efficacy of Three Different Fixed Timed Artificial Insemination Protocols in Sahiwal Cows . The Indian Journal of Animal Reproduction, 42(2), 64–73. https://doi.org/10.48165/ijar.2021.42.2.11
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