Effect of Artificial Insemination Timing on Conception Following Use of Sex sorted Semen in Sahiwal Heifers
DOI:
https://doi.org/10.48165/ijar.2026.47.01.03Keywords:
Sex sorted semen, Sahiwal heifers, Artificial insemination, GnRH, Conception rateAbstract
The present study was conducted to study the effect of timing of Artificial Insemination (AI) and the effect of GnRH on day 5 after estrus on conception rate using sex sorted semen in Sahiwal heifers. A total of forty (40) Sahiwal heifers were selected and divided into four groups (10 animals in each group) as control group C (AI was performed after 13 to 14 hours of onset of estrus), Treatment group T1 (AI was performed after 16 to 18 hours of onset of estrus), Treatment group T2 (AI was performed after 22 to 24 hours of onset of estrus) and Treatment group T3 (AI was performed after 16 to 18 hours of onset of estrus followed by administration of 20 mcg GnRH intramuscularly on day 5 of estrus after AI ). Pregnancy diagnosis was done at day 60 after AI using ultrasonography in all groups. In the control group (C), 30% conception rate was obtained, which was the lowest among all 4 groups. On the other hand, in group T1 and T2, a sim ilar conception rate (40%) was achieved. Surprisingly treatment group (T3) showed the highest conception rate (80%) in Sahiwal heifers supporting dual beneficial effect of delayed AI and GnRH on conception rate. The overall conception rate was recorded as 47.50% in 40 Sahiwal heifers under the study. The results of the present study on limited number of Sahiwal heifers demonstrated that delaying AI for 3-4 hours than conventional semen AI, along with administration of 20 mcg GnRH intramuscularly on day 5 of estrus after AI is beneficial for improving conception rate using sexed semen. Further relevant studies on a larger population size shall validate its better use in future.References
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