INTRAUTERINE PROTEOLYTIC ENZYME THERAPY AMELIORATES CERVICAL MUCUS CHARACTERISTICS AND FERTILITY IN BUFFALOES SUFFERING CLINICAL ENDOMETRITIS
DOI:
https://doi.org/10.48165/ijar.2024.45.01.5Keywords:
Clinical endometritis, Mucus score, Proteolytic enzymes, SpinnbarkeitAbstract
A total of 30 pleuriparous buffaloes with signs of clinical endometritis were randomly divided into three groups of ten animals each. The buffaloes of Group I were administered intra uterinely with a single dose of proteolytic enzymes. Group II buffaloes were treated with ceftiofur sodium and Group III animals received no treatment. The cervical mucus was collected from the buffaloes of all groups before and after treatment during estrus for assessment of cervical mucus characteristics. After treatment, the consistency of cervical mucus was thin in 80, 60 and 20 per cent of buffaloes and mucus score of discharge was found to be clear in 90, 70 and 0 per cent in Group I, II and III buffaloes, respectively. The mean spinnbarkeit values of cervical mucus in Group I, II and III before the treatment were 7.24±0.79, 7.23±0.82 and 7.09±0.89 cm, and after the treatment were 15.79±0.64, 12.90±0.71 and 7.33±0.90 cm respectively. The mean pH values of Groups I, II and III before the treatment were 8.22±0.08, 8.12±0.10 and 8.13±0.07, respectively and after treatment, 7.10±0.03, 7.43±0.14 and 8.01±0.09, respectively. The difference in the mean spinnbarkeit values and pH were significant (p<0.05) in Group I and II in pre and post-treatment and between groups. Highest improvement in pH was observed in Group I animals treated with proteolytic enzymes followed by Group II and III. The first service conception rate was highest in Group I (44.44%) followed by Group II (42.85%) and Group III (0%). It can be concluded that intrauterine infusion of proteolytic enzymes may improve cervical mucus properties and fertility in buffaloes with clinical endometritis.
References
Chenault, J. R., McAllister, J. F., Chester, S. T., Dame, K. J., Kausche, F. M. and Robb, E. J. (2004). Efficacy of ceftiofur hydrochloride sterile suspension administered parenter ally for the treatment of acute postpartum metritis in dairy cows. J. Ame. Vet. Med. Assoc., 224(10): 1634-1639.
Drillich, M., Arlt, S., Kersting, S., Bergwerff, A. A., Scherpenisse, P. and Heuwieser, W. (2006). Ceftiofur derivatives in serum, uterine tissues, cotyledons, and lochia after fetal membrane retention. J. Dai. Sci., 89(9): 3431-3438.
Drillich, M., Raab, D., Wittke, M. and Heuwieser, W. (2005). Treatment of chronic endometritis in dairy cows with an intrauterine application of enzymes: A field trial. Theriogenology, 63(7): 1811-1823.
Gulia, N., Honparkhe, M., Bisla, A., Singh, A. K. and Singh, P. (2022). Intrauterine proteolytic enzymes therapy hastens expulsion of fetal membranes in dystocia affected buffa loes. Iranian. J. Vet. Res. 23(2): 163-168.
Kadam, P. D., Markandeya, N. M., Kumawat, B. L., Sawale, A. G., Lawange, S. R. and Anbhule, R. S. (2019). Efficacy of neem seed extract±ashwagandha for the treatment of endometri tis in buffaloes. Indian J. Anim. Reprod., 40(1): 38-41.
Kaufmann, T. B., Westermann, S., Drillich, M., Plöntzke, J. and Heuwieser, W. (2010). Systemic antibiotic treatment of clin ical endometritis in dairy cows with ceftiofur or two doses of cloprostenol in a 14-d interval. Anim. Reprod. Sci., 121(1- 2): 55-62.
Kruger, M. (1999). Studies on the influence of the proteo lytic enzymes trypsin, chymotrypsin and papain on udder-pathogenic microorganisms. Vet. Prac., 27: 207-215.
Kumar, H., Bhooshan, N., Dass, R. S., & Garg, A. K. (2013). Effect of treatment of immunomodulators on recovery and conception rate in endometritic buffaloes. Indian J. Anim. Sci., 83(2): 129-132.
Kumar, P., Srivastava, S. K., Rawat, M., Yadav, M. C. and Kumar, H. (2004). Effect of certain immunomodulators on uterine infections and fertility in postpartum buffaloes. Asian-Aust. J. Anim. Sci., 17(7): 930-935.
LeBlanc, S. J., Duffield, T. F., Leslie, K. E., Bateman, K. G., Keefe, G. P., Walton, J. S. and Johnson, W. H. (2002). Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows. J. Dai. Sci., 85(9): 2223-2236.
Markusfeld, O. (1984). Factors responsible for post treatment metritis in dairy cattle. Vet. Rec., 114: 539-542.
Sharma, P. and Srivastava, S. (2018). Comparative efficacy of cef tiofur sodium, gentamicin sulfate and zingiber officinale on
physical characteristics of cervical mucus in endometritic buffaloes. Haryana Vet., 57(2): 226-228.
Sharma. S. K., Sharma, H., Dhami, A. J. and Bhong, C. D. (2008). Physico-microbial properties of cervico-vaginal mucus and its antibiotic sensitivity pattern in repeat breeding buffa loes. Indian J. Anim. Reprod., 29(1): 19-26.
Sheldon, I. M. and Dobson, H. (2004). Postpartum uterine health in cattle. Anim. Reprod. Sci., 82: 295-306.
Silva-López, R.E. and Gonçalves, R.N., 2019. Therapeutic prote ases from plants: biopharmaceuticals with multiple applica tions. J. Appl. Biotechnol. Bioeng., 6(2), pp.101-109.
Singh, H., Brar, P. S., Singh, N., Jan, M. H., Honparkhe, M. and Dhindsa, S. S. (2020). Effects of intra-uterine infusion of proteolytic enzymes on selected cytokine concentrations, uterine inflammation, and fertility in postpartum water buffalo cows with subclinical endometritis. Anim. Reprod. Sci., 215: 1-9.
Singh, J., Honparkh, M., Ghuman, S. P. S., Kumar, A., Dhindsa, S. and Chandra, M. (2017). Intrauterine proteolytic enzyme therapy for subclinical endometritis in dairy cattle. Indian J. Anim. Reprod., 38(1): 1-3.
Snedecor, G. W. and Cochran, W. G. (1989). Statistical methods, 8thEdn. Ames: Iowa State Univ. Press Iowa, 54: 71-82. Venkatesh, D., Venkataramana, K., Ramsingh, L. and Swathi, B.
(2021). Diagnosis of clinical endometritis in graded Murrah buffaloes. Pharm. Inno. J., 10(11): 219-220.
Verma, K. K., Prasad, S., Kumaresan, A., Mohanty, T. K., Layek, S. S., Patbandha, T. K. and Chand, S. (2014). Characterization of physico-chemical properties of cervical mucus in rela tion to parity and conception rate in Murrah buffaloes. Vet. World., 7(7): 467-471.
Zakiuddin, M., Tandle, M. K., Usturge, S. M., Patil, N. A., Kumar, D. D., Kasaralikar, V. R. and Suranagi, M. D. (2022). Physical properties of estrual mucus in repeat breeder buffaloes. Pharm. Inno. J., 11(10): 2066-2069.
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