Proteomic analysis of soybean cyst nematode
DOI:
https://doi.org/10.48165/Keywords:
soybean, chromatography - mass spectrometry, electrophoresis (Abstract
The soybean cyst nematode (SCN, Heterodera expression in SCN during its life cycle from J2 glycines) is the major pest of soybean in the to adult. In recent years, the application of U.S. Infections of soybean by SCN include proteomic tools such as two-dimensional chlorosis, root necrosis, loss in seed yield and polyacrylamide gel electrophoresis (2D suppression of growth of root and shoots. PAGE), matrix-assisted laser desorption Chemical control for soybean is not normally /ionization time of flight mass spectrometry used because economic and environmental ( M A L D I - T O F - M S ) , a n d l i q u i d costs of the few effective treatments are chromatography - mass spectrometry (LC-MS) prohibitive.
References
Jaubert S, Ledger TN, Laffaire JB, Piotte C, Abad P, Rosso MN. 2002. Molecular and Biochemical Parasitology 121: 205-11.
Kamm KE, Stull JT. 2011. Journal of Biological Chemistry 286: 9941-47.
Kovaleva ES, Masler EP, Subbotin SA, Chitwood DJ. 2005. Journal of Nematology 37: 292-96.
Kovaleva ES, Subbotin SA, Masler EP, Chitwood DJ. 2005. Comparative Parasitology 72: 39-49.
Laemmli UK. 1970. Nature 227: 680-85.
Matthews BF, MacDonald MH, Thai VK, Tucker ML. 2003. Journal of Nematology 35: 252-258.
Natarajan SS, Xu C, Caperna TJ, Garrett WM. 2005. Analytical Biochemistry 342: 214-20.
Perkins DN, Pappin DJC, Creasy DM, Cottrell JS. 1999. Electrophoresis 20: 3551-67.
Platzer EG, Wang W, Thompson SN, Borchardt DB. 1999. Journal of Parasitology 85: 603-07.
Sutherland JD, Witke W. 1999. Current Opinion in Cell Biology 11: 142-51.
