GREEN SYNTHESIS OF COPPER NANOPARTICLES AND THEIR EFFICACY AGAINST PLANT PATHOGENS
Keywords:
Green synthesis, Copper nanoparticles, Particle Size Analyzer, Atomic Force Microscope, Phytotoxicity and Plant pathogens.Abstract
Green synthesis of copper nanoparticles (CuNPs) and its use as antimicrobial activities against plant pathogens is a novel research area. Presently research was undertaken on synthesis of CuNPs from L. leaves Eucalyptus globules as a reducing agent and copper sulphate as precursor. Synthesized copper nanoparticles were characterized by Particles Size Analyzer (PSA) and Atomic Force Microscope (AFM) which showed the size of 30-50 nm. Such confirmed nanoparticles were evaluated against (Loose smut of wheat) Ustilago nuda tritici and (Turcicum leaf blight) by spore germination method Exserohilum turcicum which showed 100 per cent spore inhibition over the control at 50 and 100 ppm respectively. However the synthesized copper nanoparticles were failed to inhibit the mycelial growth of and by poison Sclerotium rolfsii Macrophomina phaseolina food technique. Further, CuNPs did not show any phytotoxicity symptoms on tomato seedlings by spot application on leaves under glasshouse condition.
References
Banik, S., & Sharma, P. (2011). Plant pathology in the era of nanotechnology. Indian Phytopathology, 64(2), 120–127.
Chikkanna Swamy, V., Nargund, V. B., Byadgi, A. S., Patil, R. R., & Savalgi, V. P. (2016). Green synthesis of copper nanoparticles and their efficacy against Exserohilum turcicum causing leaf blight of maize. In Proceedings of the National Symposium on Recent Advances in Plant Health Management for Sustainable Productivity (December 15–16, University of Agricultural Sciences, Dharwad, Karnataka, India, p. 139).
Jayalakshmi, & Yogamoorthi, A. (2014). Green synthesis of copper oxide nanoparticles using aqueous extract of Cassia alata flowers and particle characterization. International Journal of Nanomaterials and Biosystems, 4(4), 66–71.
Khan, P., Audil, R., Rafia, Y., & Chong, R. (2016). A chemical reduction approach to the synthesis of copper nanoparticles. International Nano Letters, 6, 21–26.
Kulkarni, P., Kulkarni, V. D., & Suryawanshi, S. (2015). Biosynthesis of copper nanoparticles using aqueous extract of Eucalyptus plant leaves. Current Science, 109(2), 255–257.
Manjunatha, S. B., Biradar, D. P., & Aladakatti, Y. R. (2016). Nanotechnology and its applications in agriculture: A review. Journal of Farm Sciences, 29(1), 1–13.
Nargund, V. B., Chikkanna Swamy, V., Giri, M. S., Hulagappa, & Ramanagouda, H. (2016). Green nanotechnology: Synthesis, characterization of silver and copper nanoparticles and their efficacy against major pathogens of maize and pomegranate. Indian Phytopathology, 69(4 Suppl.), 598–601.
Parisi, C., Vigani, M., & Cerezo, E. R. (2015). Agricultural nanotechnologies: What are the current possibilities? Nano Today, 10, 124–127.
Saranyaadevi, K., Subha, V., Ravindran, R. S., & Renganathan, S. (2014). Synthesis and characterization of copper nanoparticles using Capparis zeylanica leaf extract. International Journal of Chemical Technology Research, 6(10), 4533–4541.
Yallappa, S., Manjanna, J., Sindhe, M. A., Satyanarayan, N. D., Pramod, S. N., & Nagaraja, K. (2013). Microwave-assisted rapid synthesis and biological evaluation of stable copper nanoparticles using Terminalia arjuna bark extract. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 110, 108–115.
