CULTURAL, MORPHOLOGICAL AND MOLECULAR VARIABILITY IN COLLETOTRICHUM GLOEOSPORIOIDES COLLECTED FROM DIFFERENT FRUIT CROPS
Keywords:
fruit crops, cultural, molecular variability Colletotrichum gloeosporioides,Abstract
Anthracnose ( Penz) is the most Colleotrichum gloeosporioides important disease contributes significantly to pre-harvest and post-harvest losses in different fruit crops. Eight isolates of were Colleotrichum gloeosporioides collected from different fruit crops and characterised for cultural, morphological and molecular varians. All isolated from fruit crop hosts showed C. gloeosporioides variability amongst them. The test isolate on basic culture medium, potato dextrose agar shows significant variation in colony diameter, type of growth, colour and pigmentation. All isolates produced cylindrical hyaline conidia and they exhibited significant variability in respect of conidial dimension and size of setae. RAPD PCR analysis of the eight isolates using OPA primer series revealed that the isolate CgMg (Mango) and CgPg (Pomegranate) were closely related with 87.80 per cent genetic similarity and distinct from CgCa (Custard apple) isolate.
References
Abang, M. M., Winter, S., Green, T. K. R., Hoffmann, P., Mignouna, H. D., & Wolf, G. A. (2002). Molecular identification of Colletotrichum gloeosporioides causing yam anthracnose in Nigeria. Plant Pathology, 51, 63–71.
Bernstein, B., Zehr, E. I., Dean, R. A., & Shabi, E. (1995). Characteristics of Colletotrichum species from peach, apple, pecan, and other hosts. Plant Disease, 79, 478–482.
Cai, L., Hyde, K. D., Taylor, P. W. J., Weir, B. S., Waller, J., Abang, M. M., Zhang, J. Z., Yang, Y. L., Phoulivong, S., Liu, Z. Y., Prihastuti, H., Shivas, R. G., McKenzie, E. H. C., & Johnston, P. R. (2009). A polyphasic approach for studying Colletotrichum. Fungal Diversity, 39, 183–204.
Ekbote, S. D., Padaganur, G. M., Patil, M. S., & Chattannavar, S. N. (1997). Cultural and nutritional aspects of Colletotrichum gloeosporioides, the causal organism of mango anthracnose. Journal of Mycology and Plant Pathology, 27(2), 229–230.
Freeman, S., Katan, T., & Shabi, E. (1998). Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease, 82, 596–605.
Gautam, A. K., Avasthi, S., & Bhadauria, R. (2012). Additions to new plant fungal diseases in the 21st century in India: An update (2000–2011). Lambert Academic Publishing.
Gupta, V. K., Pandey, A., Kumar, P., Pandey, B. K., Gaur, R. K., Bajpai, V., Sharma, N., & Sharma, S. (2010). Genetic characterization of mango anthracnose pathogen Colletotrichum gloeosporioides using RAPD analysis.
Holliday, P. (1980). Fungal diseases of tropical crops. Cambridge University Press.
Hyde, K. D., Cai, L., Cannon, P. F., Crous, P. W., Damm, U., Goodwin, P. H., Chen, H., Johnston, P. R., Jones, E. B. G., Liu, Z. Y., McKenzie, E. H. C., Moriwaki, J., Noireung, P., Pennycook, S. R., Pfenning, L. H., Prihastuti, H., Sato, T., Shivas, R. G., Tan, Y. P., Taylor, P. W. J., Weir, B. S., Yang, Y. L., & Zhang, J. Z. (2009). Colletotrichum: Names in current use. Fungal Diversity, 39, 147–183.
Jaccard, P. (1908). Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles, 44, 223–270.
Jeffries, P., Dodd, J. C., Jeger, M. J., & Plumbley, R. A. (1990). The biology and control of Colletotrichum species on tropical fruit crops. Plant Pathology, 39, 343–366.
Joshi, M. K. (2008). Variability studies on Colletotrichum gloeosporioides infecting fruit crops (Doctoral dissertation, Mahatma Phule Krishi Vidyapeeth, Rahuri).
Litz, R. E. (1997). The mango: Botany, production and uses (pp. 257–280). CAB International.
Lu, Z. G., Cannon, P. F., Reid, A., & Simmons, C. M. (2004). Diversity and molecular relationships of endophytic Colletotrichum isolates from the Iwokrama Forest Reserve, Guyana. Mycological Research, 108, 53–63.
Mordue, J. E. M. (1971). Descriptions of pathogenic fungi and bacteria (No. 315). Commonwealth Mycological Institute.
Nicholson, R. L., & Moraes, W. B. C. (1980). Survival of Colletotrichum graminicola: Importance of the spore matrix. Phytopathology, 70, 255–261.
Prasanna Kumar, M. K. (2001). Management of postharvest diseases of mango (Mangifera indica) (Master’s thesis, University of Agricultural Sciences, Dharwad).
Ratanacherdchai, K., Wang, H. K., Lin, F. C., & Soytong, K. (2007). RAPD analysis of Colletotrichum species causing chilli anthracnose disease in Thailand. Journal of Agricultural Technology, 3, 211–219.
Rohlf, F. J. (1998). NTSYS-pc: Numerical taxonomy and multivariate analysis system (Version 2.0). Applied Biostatistics.
Sneath, P. H. A., & Sokal, R. R. (1973). Numerical taxonomy. W. H. Freeman.
Sutton, B. C. (1980). The coelomycetes. CAB International.
Talhinhas, P., Sreenivasaprasad, S., Neves-Martins, J., & Oliveira, H. (2005). Molecular and phenotypic analysis of diverse Colletotrichum acutatum groups. Applied and Environmental Microbiology, 71(6), 2987–2998.
Than, P. P., Prihastuti, H., Phoulivong, S., Taylor, P. W. J., & Hyde, K. D. (2008). Chilli anthracnose disease caused by Colletotrichum species. Journal of Zhejiang University Science B, 9(10), 764–778.
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18, 6531–6535.
