SEQUENCING OF FUNGAL PATHOGEN ERYSIPHE POLYGONI DC CAUSING POWDERY MILDEW OF BLACK GRAM
Keywords:
Black gram , Powdery mildew , Molecular characterization Erysiphe polygoni,Abstract
Black gram ( (L.) Hepper) is the third most important pulse Vigna mungo crop in India. Powdery mildew caused by DC is one of the major Erysiphe polygoni constraints in the production of black gram causing both qualitative and quantitative loss of grains. The fungus has very wide host range spread by air borne and oidia also produces the sexual fruiting bodies cleistothecia and difficult to differentiate the species morphologically. Present study was carried out through DNA extraction followed by Polymerase chain reaction and Agarose gel electrophoresis and through ITS rDNA Sequencing. Molecular characterization has been done through amplification of ITS1 and ITS4 region and DNA sequencing. Dharwad isolate was proved to be after blasting the gene sequences in NCBI BLAST Erysiphe polygoni programme and it had 97% homology with Mexico isolate, whereas, it was similar up to 93% with Iran isolate. Present study also approves that rDNA-ITS sequences is the best method to identify the strains.References
Alvaro, M. R., Almeida, B., Eliseu, F. P., Fernanda, R. R., Silvana, M., Paula, Z. R., Ribeiro, D., & Cesar, A. S. (2008). Characterization of powdery mildew strains from soybean, bean, sunflower, and weeds in Brazil using rDNA-ITS sequences. Tropical Plant Pathology, 33(1), 20–26.
Anonymous. (2011). Area, production, productivity of major pulses. Agropedia.
Channaveerresh, T. S., & Kulkarni, S. (2014). In vitro evaluation of fungicides, bioagents and botanicals against Erysiphe polygoni DC. in black gram. Research Journal of Agricultural Sciences, 5(4), 798–801.
Channaveerresh, T. S., Kulkarni, S., & Vijaykumar, A. G. (2014). Evaluation of black gram genotypes for resistance to powdery mildew caused by Erysiphe polygoni DC. Karnataka Journal of Agricultural Sciences, 27(1), 85–87.
de Candolle. (1802). History and taxonomy. In D. M. Spencer (Ed.), The powdery mildews (pp. 3–15). Academic Press.
Keiko, U., Susumu, T. S. M., Kazuhiro, S., & Yukio, S. (2009). Morphological and molecular characterization of Oidium subgenus Reticuloidium (powdery mildew) newly occurred on cucumber in Japan. Journal of General Plant Pathology, 75, 92–100.
Linnaeus, C. (1767). History and taxonomy. In D. M. Spencer (Ed.), The powdery mildews (pp. 16–25). Academic Press.
Rangaswami, G. (1988). Diseases of crop plants in India. Prentice Hall of India.
Sachan, S. N. (1991). Phonological spectrum and perennation of powdery mildew fungi of Doon valley—I—Erysiphe. Indian Phytopathology, 44, 121–123.
Schnathorst, W. C. (1965). Environmental relationships in the powdery mildews. Annual Review of Phytopathology, 3, 343–366.
Sivaprakasam, K., Marimuthu, T., Radhakrishnan, T., & Vairavan, K. (1981). Influence of dates of sowing and spacing on the incidence of powdery mildew in black gram. Madras Agricultural Journal, 68, 65–67.
White, T. J., Bruns, T., Lee, S., & Taylor, J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). Academic Press.
Yarwood, C. E. (1936). Tolerance of Erysiphe polygoni and certain other powdery mildew to low humidity. Phytopathology, 26, 845–859.
