Effective treatment regimen for control and eradication  of oxyurids in laboratory rodents

Krishnaveni  N; Sandhya  S; Rosa  J S; Shrruthi B M; Ramachandra S G

doi:10.48165/jlas.2019.2.1.7

Authors

Krishnaveni N Central Animal Facility, Indian Institute of Science, Bangalore 560 012 Author
Sandhya S Central Animal Facility, Indian Institute of Science, Bangalore 560 012 Author
Rosa J S Central Animal Facility, Indian Institute of Science, Bangalore 560 012 Author
Shrruthi B M Central Animal Facility, Indian Institute of Science, Bangalore 560 012 Author
Ramachandra S G Central Animal Facility, Indian Institute of Science, Bangalore 560 012 Author

DOI:

https://doi.org/10.48165/jlas.2019.2.1.7

Keywords:

Oxyurids, rodents, ivermectin, piperazine

Abstract

Pinworm infestation is more common in most conventional colonies of laboratory rodents. They usually do not exhibit any clinical signs and no adverse effects have been reported on reproduction in infested animals, although, there is an inverse relationship between bodyweight and degree of infestation in newly weaned rodents. It has been reported that oxyurid infestations influence experimental results and also interfere with research goals in number of ways. The prevalence of pinworms in rodent population depends on many factors, including environmental load, gender, age, strain, and immune status. Unlike many common viral diseases of laboratory mice, pinworm infestations can be treated. A rederivation program is highly recommended if other pathogens are present in the colony. Present study was carried out to evaluate the effective treatment regimen for oxyurid infestation. Rodent colonies were screened for adult oxyurid worms or eggs by cellophane tape or flotation method. This screening revealed the presence of pinworm in mouse and rat colonies. Treatment regimen included administration of combinations of fenbendazole and praziquantel for group I at the rate of 150 mg/kg and 50 mg/kg respectively for 24 days through drinking water. Piperazine and ivermectin was administered for group II at the rate of 10.8 mg/mL for 14 days and 0.01mg/mL for next 14 days respectively through drinking water. No oxyurids were found in the colony after 28 days of treatment in group II. It was concluded based on the treatment regimen that piperazine and ivermectin combination was more effective in complete eradication of oxyurids in rodents.

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References

Battles AH, Adams SW, Courtney CH, Mladinich CRT (1987). Efficacy of ivermectin against natural infection of Syphacia muris in rats. Lab. Anim. Sci. 37: 791-792.

Blair LS, Thompson PE, Vandenbelt JM (1968). Effects of pyrvinium pamoate in the ration or drinking water of mice against pinworms Syphacia obvelata and Aspiculuris tetraptera. Lab. Anim. Care. 18: 314-327.

Eguiluz C, Viguera E, Perez J (2001). Modification of the anal tape method for detection of pinworms in rodents. Lab. Anim. 30: 54-55.

FELASA, Federation of European Laboratory Animal Science Associations. http://www.felasa.eu/

Flynn RJ (1973). Parasites of laboratory animals. Iowa State University Press, pp. 239-242.

Goncalves L, Pinto R M, Vicente JJ, Noronha D, Gomes DC (1998). Helminths parasites of conventionally maintained laboratory mice. II. Inbred strains with an adaptation of the anal swab technique. Mem. Inst. Oswaldo Cruz. 93: 121-

126.

Graham C F (1941). A device for the diagnosis of Enterobius infection. Am. J. Trop. Med.. 21: 159-161.

Habermann RT, Williams FP (1958). The identification and control of helminths in laboratory animals. J. Natl. Cancer Inst. 20: 979-1009.

Harikrishnan VS, Ranaraj V R, Sreeja K R, Fernandez AC (2011). Eradication of pinworms (Syphacia spp.) in a laboratory rodent colony. J. Lab. Anim. Sci. 1(1): 64-67.

Huerkamp M J (1993). Ivermectin eradication of pinworms from rats kept in ventilated cages. Lab. Anim. Sci. 43:86-90.

Huerkamp MJ, Benjamin KA, Zitzow LA, Pullium J K, Lloyd J A, Thompson W D, Webb SK, Lehner N D (2000). Fenbendazole treatment without environmental decontamination eradicates Syphacia muris from all rats in a large, complex research institution. Contemp. Top. Lab. Anim. Sci. 39(3): 9-12.

Jacobs AH (1942). Enterobiasis in children. Incidence, symptomology, and diagnosis, with a simplified Scotch cellulose tape technique. J. Pediat. 21: 497-503.

Kantee H (1973). Piperazines for the elimination of pinworms and roundworms. Sairaanhoitaja 49:26.

Kathleen RP, Johnston NA (2002). A review of treatments for the eradication of pinworm infections from laboratory rodent colonies. J. Am. Asso. for Lab. Anim. Sci. 41(2): 36- 46.

Klement P, Augustine JM, Delaney KH, Klement G, Weitz JI (1996). An oral ivermectin regimen that eradicate pinworms (Syphacia spp.) in laboratory rats and mice. Lab. Anim. Sci. 46: 286-290.

Lipman N S, Dalton SD, Stuart A R, Arruda K (1994). Eradication of pinworms (Syphacia obvelata) from a large mouse breeding colony by combination oral anthelmintic therapy. Lab. Anim. Sci. 44: 517-520.

MacArthur J A, Wood M (1978). Control of oxyurids in mice using thiabendazole. Lab. Anim. 12: 141-143.

Martin R J (1997). Modes of action of anthelmintic drugs. Vet. J. 154:11-34.

Morrell J M (1999). Techniques of embryo transfer and facility decontamination used to improve the health and welfare of transgenic mice. Lab. Anim. 33:201-206.

Oge H, Ayaz E, Ide T, Dalgic S (2000). The effect of doramectin, moxidectin and netobimin against natural infections of Syphacia muris in rats. Vet. Parasitol. 88: 299-303.

Owen D, Turton J A (1979). Eradication of the pinworm Syphacia obvelata from an animal unit by anthelmintic therapy. Lab. Anim. 13:115-118.

Pritchett K R, Johnston NA (2002). A review of treatments for the eradication of pinworm infections from laboratory rodent colonies. Contemp. Top. Lab. Anim. Sci., 41: 36-46.

Reiss C S, Herrman JM, Hopkins R E (1987). Effect of anthelminthic treatment on the immune response of mice. Lab. Anim. Sci. 37: 773-775.

Stahl W (1961). Syphacia muris, the rat pinworm. Sci. 133: 576-577.

Taffs L F (1976). Pinworm infections in laboratory rodents: a review. Lab. Anim. 10: 1-13.

Taylor DM (1992). Eradication of pinworms (Syphacia obvelata) from Syrian hamsters in quarantine. Lab. Anim. Sci. 42: 413-414.

Unay E S, Davis B J (1980). Treatment of Syphacia obvelata in the Syrian hamster (Mesocricetus auratus) with piperazine citrate. Am. J. Vet. Res. 41: 1899-1900.

Wagner J E (1970). Control of mouse pinworms, Syphacia obvelata, utilizing dichlorvos. Lab. Anim. Care. 20: 39-44.

West W L, Schofield JC, Bennett B T (1992). Efficacy of the “micro-dot” technique for administering topical 1% ivermectin for the control of pinworms and fur mites in mice. Contemp. Top. Lab. Anim. Sci. 31:7-10.

Zenner L (1998). Effective eradication of pinworms (Syphacia muris, Syphacia obvelata and Aspiculuris tetraptera) from a rodent breeding colony by oral anthelmintic therapy. Lab. Anim. 323: 337-342.