Mouse milk somatic cell count in coagulase negative  Staphylococcus species induced mastitis

Krishnamoorthy  P; Satyanarayana  M L; Shome B R; Rahman H

doi:10.48165/jlas.2019.2.1.3

Authors

Krishnamoorthy P M.V.Sc., FASAW Scientist, Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS) IVRI Campus, Bellary Road, Hebbal, Bangalore-560024, Karnataka, India. Author
Satyanarayana M L Department of Veterinary Pathology, Veterinary College, Hebbal, Bangalore-560024, Karnataka, India Author
Shome B R Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), IVRI Campus, Hebbal, Bangalore-560024, Karnataka, India Author
Rahman H Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), IVRI Campus, Hebbal, Bangalore-560024, Karnataka, India Author

DOI:

https://doi.org/10.48165/jlas.2019.2.1.3

Keywords:

epidermidis, chromogenes, haemolyticus

Abstract

Bovine mastitis is an economically important disease of bovines and caused by multi etiological factors in which bacteria is the major cause. In the present study, milk somatic cell count (SCC) in mouse mastitis induced by Staphylococcus epidermidis, S. chromogenes, S. haemolyticus and S. aureus isolated from apparently normal bovine milk was studied. The 2 x 104 cfu organisms in 50 µl per teat were inoculated through intramammary route in 4th and 5th pairs of abdominal mammary gland in mice. The squeeze method, a technique was developed and standardized for milk collection from mice at 6, 12, 24, 48, 72 and 96 hr after intramammary inoculation (IMI). The milk somatic cell count was estimated using NucleoCounter SCC-100®. The mouse milk ranging from 50 to 200 µl per mice was collected successfully. The milk somatic cell count showed significant increase in S. aureus inoculated mice at 6, 12, 24 hr and increase was two to four folds when compared to PBS control. The three coagulase negative staphylococcus species showed increased SCC levels at 24, 48, 72 and 96 hr after IMI. Thus, Coagulase negative staphylococci (C) species can increase the mice milk somatic cell count moderately but two to four fold increase was observed in S. aureus which indicated the subclinical nature of CNS infections in animals. Mouse is a suitable model to study coagulase negative staphylococcus species induced bovine subclinical mastitis.

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