Pathological changes in the mammary gland of  rabbits following intra-mammary inoculation of  Mycoplasma agalactiae

Uma S; Narayanaswamy  H D; Sathyanarayana  M L; Gajendragad M R; S G Ramachandra; Krishnaveni  N

doi:10.48165/jlas.2019.2.2.4

Authors

Uma S Department of Veterinary Pathology, Veterinary College, Hebbal, Bangalore, India Author
Narayanaswamy H D Department of Veterinary Pathology, Veterinary College, Hebbal, Bangalore, India Author
Sathyanarayana M L Department of Veterinary Pathology, Veterinary College, Hebbal, Bangalore, India Author
Gajendragad M R Project Directorate on Animal Disease Monitoring and Surveillance, Hebbal, Bangalore, India Author
S G Ramachandra Central Animal Facility, Indian Institute of Science, Bangalore, India Author
Krishnaveni N Department of Veterinary Pathology, Veterinary College, Hebbal, Bangalore, India Author

DOI:

https://doi.org/10.48165/jlas.2019.2.2.4

Keywords:

Contagious agalactia, Mycoplasma agalactiae, mastitis, rabbit, mammary gland

Abstract

Contagious agalactia is a disease predominantly of milking sheep and goats caused mainly by Mycoplasma agalactiae, clinically manifested as mastitis, arthritis, keratoconjunctivitis and pneumonia. Pathological changes in the mammary gland of rabbits following intra-mammary inoculation of M. agalactiae were studied. Clinical mastitis developed within 24 hpi. It was initially acute but became chronic by the end of the experiment at 9 dpi. The disease was characterized by atrophy of the infected mammary glands, resulting in marked agalactia. Histopathology revealed that the mastitis was acute and purulent initially, followed by infiltration of mononuclear cells with fibroplasia in the interacinar tissue, and later by massive fibrosis by 9 dpi.

Downloads

Download data is not yet available.

References

Anderson JC, Howard CJ and Gourlay RN (1976). Experimental Mycoplasma mastitis in mice. Infect. Immun. 13: 1205-1208.

Ball HJ, Logan EF and Campbell JN (1987). Mycoplasma californicum mastitis in ewes as an experimental model for antibiotic treatment. Epidemiol. Infect. 98: 369-378.

Bergonier D, Berthelot X and Poumarat F (1997). Contagious agalactiae of small ruminants: current knowledge concerning epidemiology, diagnosis and control. Rev.Sci. Tech. Off. Int .Epizoot. 16: 848-873.

Castro-Alonso A, De La Fe C, Espinosa De Los Monteros A et.al (2010). Chronological and immunohistochemical characterization of the mammary immunoinflammatory response in experimental caprine contagious agalactia. Vet. Immunol. Immunopathol. 136(1-2): 43-54.

Castro-Alonso A, Rodríguez F, De La Fé C et.al (2009). Correlating the immune response with the clinical– pathological course of persistent mastitis experimentally induced by Mycoplasma agalactiae in dairy goats. Res. Vet. Sci. 86: 274–280.

Darzi MM, Sood N, Gupta PP and Banga HS (1998). The pathogenicity and pathogenesis of Mycoplasma capricolum subsp. capripneumoniae (F38) in the caprine mammary gland. Vet. Res. Commun. 22: 155-165.

De La Fe C, Castro-Alonso A, Herráez P, José B and Poveda A (2010). Recovery of Mycoplasma agalactiae from the ears of goats experimentally infected by the intramammary route. Vet. J. 190(1):94-97.

Garg DN, Singh Y, Yadav R and Mahajan SK (2004). Experimental pathogenicity evaluation of Mycoplasma canadense from bovine mastitis in vitro and in vivo laboratory models. Indian J. Exp. Biol. 42:152-156.

Hasso SA, Al-Aubaidi JM and Al-DarrajiAM (1993). Contagious agalactia in goats: It’s severity as related to the route of infection and pregnancy. Small Ruminant Res., 10(3): 263-275.

Howard CJ and Taylor G (1985). Humoral and cell mediated immunity. In: Razin S, Barile M F (Eds.) The Mycoplasma Pathogenicity. The Mycoplasmas, vol. 4. Academic Press Inc., Orlando, FL, pp. 259–292

Kaur C, Garg DN and Mahajan SK (1998). Experimental mastitogenicity of Mycoplasma capricolum subsp. capripneumoniae for rabbit mammary glands. Indian J. Exp. Biol. 36: 407-410.

Misri J, Gupta PP and Ahuja SP (1988). Biochemical changes mycoplasmal in milk mastitis in experimental goats. Acta Vet. Brno. 57: 19-30.

Nicholas R (1996).Contagious agalactia: an update. In: Frey J and Sarris K: Agriculture and Biotechnology, Mycoplasmas of Ruminants: Pathogenicity, Diagnostics, Epidemiology and Molecular Genetics. Luxembourg, pp 172.

Nicholas R A J (2002). Improvements in the diagnosis and control of diseases of small ruminants caused by mycoplasmas. Small Ruminant Res. 45: 145–149.

Rana JS, Gupta PP, Banga HS and Kumar D (1992). Caprine mastitis experimentally induced with mycoplasma ovine/ caprine serogroup 11 (2-D). Acta Vet. Brno. 61: 231-241.

Razin S, Yogev D and Naot Y (1998). Molecular biology and pathogenicity of mycoplasmas. Microbiol. Mol. Biol. Rev, 62: 1094–1156.

Reilly G A C, Kennedy S, Mcaliskey M and Ball HJ (1993). The pathology of experimental M y c o p l a s m a californicum mastitis in ewes. J. Comp. Path. 109: 155-161.

Rottem S (2003). Interaction of Mycoplamas with host cells. Physiol. Rev. 83: 417-432.

Sanchis R, Abadie G, Lambert M et.al (2000). Inoculation of lactating ewes by the intramammary route with Mycoplasma agalactiae: comparative pathogenicity of six field strains. Vet. Res. 31: 329 – 337.

Taoudi A, Johnson DW, Kheyyali D and Kirchhoff H (1987). Pathogenicity of Mycoplasma capricolum in sheep after experimental infection. Vet. Microbiol.137-144.