Detection of Pseudomonas aeruginosa infection  from the water bottles of immune-compromised  mice by conventional and PCR based microbiology

A D Shinde; A D Ingle

doi:10.48165/jlas.2020.3.1.1

Authors

A D Shinde Laboratory Animal Facility, ACTREC, Tata Memorial Centre Kharghar, Navi Mumbai- 410210 Author
A D Ingle Officer-in-Charge, Laboratory Animal Facility, ACTREC, Tata Memorial Centre, Kharghar, Navi Mumbai- 410210. Author

DOI:

https://doi.org/10.48165/jlas.2020.3.1.1

Keywords:

Pseudomonas aeruginosa, PCR, mice

Abstract

Microbiological control of laboratory animals is important in any animal facility for production of microbiologically clean animals. It is possible through accurate diagnosis of the rodent pathogens in the laboratory. Pseudomonas aeruginosa is a ubiquitous and free living organism which infects the gastrointestinal tract through water. Normal animals do not show clinical signs in infection but immuno-compromised/ irradiated mice show varied symptoms of the disease. In this study, Pseudomonas aeruginosa was isolated from drinking water provided to the laboratory animals. The P. aeruginosa grown on the Nutrient and MacConkey agar plates were subjected for identification by using colony characters, odor and biochemical tests. DNA was isolated and PCR was carried out by using positive DNA as positive control. PCR product of 726 bp pairs was developed on 2% agarose gel for confirmation of the organisms. PCR method along with conventional method may offer sensitive and rapid detection of P. aeruginosa in water as well as clinical specimens.

Downloads

Download data is not yet available.

References

Baker David G (1998). Natural Pathogens of Laboratory Mice, Rats, and Rabbits and Their Effects on Research, Clin.Microbiol. Rev. 11(2): 231-266.

Charles River Laboratories Model and ServicesPseudomonas aerugenosa- Technical sheet (2009). http://www. criver.com/files/pdfs/infectious-agents/rm_ld_r_ pseudomonasaeruginosa.aspx

Dieterich H, Khaschabi D, Albini B (1996). Isolation of Enterococcus durans and Pseudomonas aeruginosa in a SCID mouse colony, Lab. Anim. 30: 102-107.

Holcombe H, Schauer D (2007).Enterobacteriaceae Pseudomonas aeruginosa and Streptobacillusmoniliformis. Second edition, Edts. Fox James, Barthold Stephen, Davisson Mureil, Newcomer Christian, Quimby Fred, Smith Abigail,The Mouse in Biomedical Research Diseases. Academic Press, London: 365-387.

Homberger FR, Pataki Z, ThomannPE (1993).Control of Pseudomonas aeruginosa infection in mice by chlorine treatment of drinking water.Lab. Anim. Sci. 43: 635-637.

Ingle AD,Shinde AD (2014). Microbiological Assessment of Laboratory Rodents: Perspectives from Laboratory Animal Facility of ACTREC.J. Lab. Anim. Sci. 2: 1-9.

Jeong ES, Lee KS, Heo SH, Seo JH, Choi YK (2011).Triplex PCR for the simultaneous detection of Pseudomona aeruginosa, Helicobacter hepaticus and Salmonella typhimurium.Exp. Anim. 60, 1: 65-70.

Mahabir E, Bulian D, Bensch S, Schmidt J (2009). Elimination of P. aeruginosa in Mice by Treatment with Chlorine, and the use of Microbiological and PCR Analyses, Scand. J. Lab. Anim. Sci., 36 (4): 355-361.

Meynard J, Marguerite G, Batissel D, et al. (1999). Pseudomonas aeruginosa, infection inhuman immunodeficiency virus infected patients, J.Inf.38 (3): 176-181.

Urano T, Noguchi K, Jiang G, Tsukumi K (1995). Survey of Pseudomonas aeroginosa contamination in human beings and laboratory animals.Expt. Anim, 44: 233-239.