Refined method of blood collection in chickens using tonic immobility

Bijargi Shriharsh R; Ramesh  S; Bhaskar Vemu

doi:10.48165/jlas.2020.3.2.2

Authors

Bijargi Shriharsh R Ph.D. Scholar, Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007, INDIA Author
Ramesh S Professor and In charge, Laboratory Animal Medicine Unit, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 051, INDIA Author
Bhaskar Vemu M.V.Sc. Scholar, Department of Veterinary Pharmacology and Toxicology, Madras Veterinary College, Chennai-600 007, INDIA Author

DOI:

https://doi.org/10.48165/jlas.2020.3.2.2

Keywords:

Blood collection, Chicken, Refinement, Tonic immobility

Abstract

The domestic fowl (Gallus gallus domesticus) is a popular commercial species and is much studied for meat and egg production. Frequent studies on genetics, immunology, diagnostics and therapeutics are conducted in this species. For all these studies frequent blood collection is required. In the present study, blood collection technique in domestic fowl was standardized, wherein a single individual researcher can restrain the bird using the weak hand in the state of tonic immobility and carry out blood collection using the dominant hand, thus precluding the need for additional manpower which enhances the efficiency of collection especially for studies involving large number of birds without exposing them to much stress and discomfort. This fulfils one of the important objectives of refining experimentation by reducing stress on the birds.

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