Anti-osteoarthritic activity of   Acacia nilotica pods in Wistar rats

Anjaria  J; Ghag Sawant. M; N Shah; T  Khan; G Kaur; R Goyal; A Chowdhary

doi:10.48165/jlas.2020.3.2.1

Authors

Anjaria J SPP School of Pharmacy and Technology Management, NMIMS, Mumbai, India Author
Ghag Sawant. M Haffkine Institute for Training, Research and Testing, Mumbai, India Author
N Shah Haffkine Institute for Training, Research and Testing, Mumbai, India Author
T Khan SVKM’s Dr. Bhanuben Nanavati College of Pharmacy, Mumbai, India Author
G Kaur SPP School of Pharmacy and Technology Management, NMIMS, Mumbai, India Author
R Goyal SPP School of Pharmacy and Technology Management, NMIMS, Mumbai, India Author
A Chowdhary Grant Medical College, Sir J.J. Hospital, Mumbai Author

DOI:

https://doi.org/10.48165/jlas.2020.3.2.1

Keywords:

Acacia nilotica pods, Papain, Arthritis, TNFα & IL-10

Abstract

Osteoarthritis is one of the common joint degenerative disorders for which only symptomatic treatment is available. In the present study we have investigated anti arthritic activity of the Methanolic extracts of the pods of Acacia nilotica in papain induced osteoarthritis model which mimics the human arthritis. Osteoarthritis was induced in rats by intra articular injection of 0.8mg of papain and 0.03M of cysteine on day 0, 4 and 7. Methanol extract of A.nilotica pods was administered to the animals in the doses of 100, 200, and 400 mg/kg for 15 days after the injection of papain and cysteine. Celecoxib (30 mg/kg) was used as the reference standard drug. The joint activity was assessed by wire hang test and measurement of the knee joint diameter. At the end of 15 days blood samples were collected for the analysis of TNFα, and IL-10 gene expression by Real Time PCR. The animals were sacrificed and the knee of the each animal was collected for the histological studies of the joint.Injection of papain with cysteine produced a significant increase in joint diameter and reduction in joint strength as depicted by decrease in wire hanging time. The Methanolic extract of A.nilotica pods produced time and dose dependent decrease in swelling of the knee joint along with significant increase in joint strength. There was a significant elevation of TNF-α as well as IL-10 gene expression in the rats treated with 400 mg/kg of test extract. Celecoxib or lower doses of A. nilotica produced slight increase in these levels. Histopathological studies revealed that the reversal of damages in the cartilage induced by papain and cysteine after administration of the extract.

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References

Abramson SB, Martel-Pelletier J, Pelletier JP (2001). Osteoarthritis, an inflammatory disease: potential implication for the selection of new therapeutic targets. Arthritis and rheumatism, 44:1237-1247.

Ali A (2012). Acacia nilotica: A plant of multipurpose medicinal uses. Journal of Medicinal Plants Research. 6:1492-1496.

Chaubal R (2003). Isolation and X-ray study of an anti inflammatory active androstene steroid from Acacia nilotica. Planta Medica., 69:287-288.

Felson D (2009). Osteoarthritis. Harrison’s principles of internal medicine. pp 2828–2836

Plows D, Probert L, Georgopoulos S (1995). The role of Tumor necrosis factor (TNF) in arthritis: studies in transgenic mice. Rheumatol Eur., 51–54.

Goldring SR, Goldring MB (2006). Clinical aspects, pathology and pathophysiology of osteoarthritis. Journal of musculoskeletal & neuronal interactions.,6:376-378.

Jarullah B, Mujapara AK (2009). Analysis of the genetic diversity of arthritis patients with reference to the factors inducing its onset. Int. J Genet.,1:6-9.1:6-9.

Katz JN (2006). Total joint replacement in osteoarthritis. Best Practice & Research Clinical Rheumatology. 20:145-153.

Klein S (2012). Noninvasive in vivo assessment of muscle impairment in the mdx mouse model – A comparison of two common wire hanging methods with two different results. Journal of Neuroscience Methods., 203:292-297.

Lampio F (2013).The Anti-fertility Effects of Acacia nilotica in Male Wistar Rats. Reprod Infertil., 14(1): 39–42.

Livak KJ, Schmittgen TD (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods., 25(4):402-408.

Mahajan A, Tandon V, Verma S (2005). Osteoarthritis. Journal of the association of physicians of

India.53:634-641.

Murat N, Karadam B, Ozkal S, (2007). Quantification of papain-induced rat osteoarthritis in relation to time with the Mankin score. Acta orthopaedica et traumatologica turcica. 41(3):233-237.

Raghavendra MP, Raveesha KA, Satish S (2006). In vitro evaluation of anti-bacterial spectrum and phytochemical analysis of Acacia nilotica spectrum. Journal of Agricultural Technology 2:77-88.

Ramalingam K (2009). Musculo-skeletal joint inflammatory diseases-New vistas from animal model studies–An overview. Journal of Biochem Tech., 1:108-113.

Saka S (2011). Anti-Diarrhoeic and antibacterial Effects of Aqueous Pod Extract of Acacia nilotica in Albino Rats water as a solvent and subjected to phytochemical. Nigerian Veterinary Journal, 32:30-35.

Sakthivel K (2012). R2 Anticancer activity of Acacia nilotica (L.) Wild. Ex. Delile subsp. indica against Dalton’s ascitic lymphoma induced solid and ascitic tumor model. Asian Pac J Cancer Prev. 13(8):3989-95.

Singh B, Singh B, Sarma B (2009). Potential

chemoprevention of N-nitrosodiethylamine-induced hepatocarcinogenesis by polyphenolics from Acacia nilotica bark.Chem Biol Interact. 181(1):20-8.

Verma V, Singh U, Sharma A, (2012). A review on Acacia nilotica Linn. and its ethnobotany, phytochemical and pharmacological profile. International journal of pharmaceutical research and development. 4:251-256.