A study on hematological, serum biochemical and  microbiological parameters in wild caught rodents

Nidhi H; Sandhya  S; Krishnaveni  N; Shrruthi M; Rosa  J S; Ramachandra  S G

doi:10.48165/jlas.2021.4.1.5

Authors

Nidhi H Central Animal Facility, Indian Institute of Science, Bangalore 560 012, Karnataka Author
Sandhya S Central Animal Facility, Indian Institute of Science, Bangalore 560 012, Karnataka Author
Krishnaveni N Central Animal Facility, Indian Institute of Science, Bangalore 560 012, Karnataka Author
Shrruthi M Central Animal Facility, Indian Institute of Science, Bangalore 560 012, Karnataka Author
Rosa J S Central Animal Facility, Indian Institute of Science, Bangalore 560 012, Karnataka Author
Ramachandra S G Central Animal Facility, Indian Institute of Science, Bangalore 560 012, Karnataka Author

DOI:

https://doi.org/10.48165/jlas.2021.4.1.5

Keywords:

serum biochemistry, hematology, microbiology, wild rodents

Abstract

Laboratory mice constitute the most popular animal models used in biomedical research today. Like all animals, even mice housed in ‘barrier’ facilities are prone to infection. Wild mice (Mus musculus) living near vivaria could serve as a source of infection or infestation in laboratory mouse colonies, although little is known about the prevalence of infectious diseases in wild mouse. Very few surveys have been conducted on wild rodent populations to determine the prevalence of viruses and bacteria and also health monitoring of the wild rodents. Pathogens (bacteria, parasites) often gain entry into colonies through the introduction of infected animals or infected animal products, but viral dissemination via contaminated fomites or local wild rodents has also been implicated in the spread of disease. Hence, a preliminary study was undertaken to determine the prevalence of rodent pathogens and health status in wild mouse populations in and around and Bangalore. The present study revealed that none of the animals tested were positive for viral pathogens. However, bacterial pathogens and parasites were found in wild caught mice. Hematological and serum biochemical parameters were comparable to the colony bred anim

Downloads

Download data is not yet available.

References

Becker SD, Bennett M, Stewart JP, Hurst JL (2006). Serological survey of virus infection among wild house mice (Mus domesticus) in the UK. Lab. Anim. 41:229–238.

Bradford SG, Leslie WY, Kenneth LH (2002). Rat and Mice: Parasitic Diseases. Laboratory Animal Medicine and Science - Series II. Fernández I, Peña A, Teso ND, Pérez V, Cuesta JR (2009). Clinical biochemistry parameters in C57BL/6J mice after blood collection from the submandibular vein and retro-orbital plexus. J. Am. Assoc. Lab. Anim. Sci. 49(2):202-206.

Ingle AD, Shinde AD, Ahire SD (2011). Hematological values of mice and rat strains: perspectives from ACTREC laboratory animal facility. J. Lab. Anim. Sci. 1:59-63.

Mazzaccara C, Giuseppe L, Sacchetti (2008). Age-related reference intervals of the main biochemical and hematological parameters in C57BL/6J, 129SV/EV and C3H/HeJ mouse strains. PLoS ONE 3(11): e3772. doi:10.1371/journal.pone.0003772

Michael JPEC, Chan S, Krieg NR (2009). Microbiology. 5th edition Tata McGraw-Hill company Limited. Pp 150-170.

Moro DM, Lloyd L, Smith AL, Shellam GR, Lawson MA (1999). Murine induced viruses in an island population of introduced house mice and endemic short tailed mice in Western Australia. J. Wildlife Dis. 35(2):301–310.

Singleton GR, Smith AL, Shellam GR, Fitzgerald N, Muller WJ (1993). Prevalence of viral antibodies and helminths in field populations of house mice (Mus domesticus) in southeastern Australia. Epidemiol. Inifect. 110: 399-417.

Smith AL, Singleton GR, Hansen GM, Shellam G (1993). Serologic survey for viruses and Mycoplasma pulmonis among wild house mice (Mus domesticus) in South Eastern Australia. J. Wildlife Dis.:219-229.

Taffs LF (1976). Pinworm infections in laboratory rodents. Lab. Anim. 10:1-13.

Timm RM (1994). Wildlife Damage Management. In : The Handbook on Prevention and Control of Wildlife Damage. Eds. Scott E. Hygnstrom, Robert M. Timm, Gary E. Larson. University of Nebraska-Lincoln, USA pp. B31-46.

Trudel L, St-Amand L, Bareil M, Cardinal P, Lavoie MC (1986). Bacteriology of the oral cavity of BALB/c mice. Can. J. Microbiol. 32(8):673-678.