Intragastric administration of radioactive tablet  under the influence of anesthesia for gastro  retentive study in New Zealand White rabbit

Y P Pawar; M Karpe; S U Awasare; T S Upadhey; A A Damle; M G R Rajan

doi:10.48165/jlas.2021.4.1.2

Authors

Y P Pawar Radiation Medicine Center, Bhabha Atomic Research Centre, Tata Memorial Centre Annex Building, Parel, Mumbai 400012, Maharashtra, India Author
M Karpe Bharati Vidyapeetha College of Pharmacy, C.B.D. Belapur, Navi Mumbai, Maharashtra, India Author
S U Awasare Radiation Medicine Center, Bhabha Atomic Research Centre, Tata Memorial Centre Annex Building, Parel, Mumbai 400012, Maharashtra, India Author
T S Upadhey Radiation Medicine Center, Bhabha Atomic Research Centre, Tata Memorial Centre Annex Building, Parel, Mumbai 400012, Maharashtra, India Author
A A Damle Radiation Medicine Center, Bhabha Atomic Research Centre, Tata Memorial Centre Annex Building, Parel, Mumbai 400012, Maharashtra, India Author
M G R Rajan Radiation Medicine Center, Bhabha Atomic Research Centre, Tata Memorial Centre Annex Building, Parel, Mumbai 400012, Maharashtra, India Author

DOI:

https://doi.org/10.48165/jlas.2021.4.1.2

Keywords:

Intragastric dosing, Tablet, Anesthesia, Scintigraphy imaging, Rabbit

Abstract

The purpose of this study was to evaluate the effect of dissociative anesthesia in the oral administration of radiolabelled gastro-retentive Metformin tablet in New Zealand White rabbits. The radiolabelled tablet formulation was administered to the rabbit by oral route. In this pilot experiment, it was observed that maximum activity was retained in the oral cavity as compared to gastric area. Hence, to address this problem modification with oral gavaging was done. Intragastric administration of radiolabelled Metformin drug formulation under effect of dissociative anesthesia resulted in 92.6% retention of radioactive tablet in the gastric region with minimal esophageal contamination. Therefore, it was possible to carry out further experiments in these animals with successful administration of tablet followed by sequential scintigraphy imaging at different time intervals. Significant retention was observed up to 6 hrs. Judicious use of dissociative anesthesia helps to attempt the practical problems for oral administration of a tablet in laboratory animals. The procedure can be useful for further pre-clinical testing of newer drug formulations in tablet form.

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