Phytochemical evaluation and HPTLC fingerprint  profile of various extracts of Cassia tora

M Thirumala; N B Shridhar; J S  Sanganal; H D N Swamy; S G Ramachandra

doi:10.48165/jlas.2023.6.2.2

Authors

M Thirumala Facility Manager, Primate Research Laboratory, Indian Institute of Science, Bengaluru 560012. Author
N B Shridhar Associate Professor, Department of Veterinary Pharmacology and Toxicology, Veterinary College, Shivamogga 577204. Author
J S Sanganal Professor and Head, Department of Veterinary Pharmacology and Toxicology, Veterinary College, Hebbal, Bengaluru 560024 Author
H D N Swamy Professor, Department of Veterinary Pathology, Veterinary College, Hebbal, Bengaluru 560024. Author
S G Ramachandra Director, ICMR-National Animal Resource Facility for Biomedical Research, Department of Health Research, Ministry of Health and Family Welfare, Govt. of India, Genome Valley, Shamirpet, Hyderabad 500101. Author

DOI:

https://doi.org/10.48165/jlas.2023.6.2.2

Keywords:

Cassia tora, Phytochemical analysis, HPTLC, Fingerprint.

Abstract

The objective of the present study is to evaluate phytochemical composition and the high-performance thin layer chromatography (HPTLC) fingerprint profile of methanol, ethanol, ethyl acetate, acetone, and aqueous extracts of medicinally useful plant Cassia tora. The CAMAG HPTLC system was used for the fingerprint profiling of various extracts of C. tora using the mobile phase toluene: ethyl acetate: glacial acetate (55: 45: 3 v/v/v). The profile showed that the various extracts of C. tora exhibited several peaks with different Rf values when visualized at 254 nm and 366 nm. The results from HPTLC fingerprint scanned at wavelength 550 nm revealed the presence of 18, 13, 15, 12 and 14 phytoconstituents in methanol, ethanol, ethyl acetate, acetone, and aqueous extracts, respectively. The result of HPTLC analysis of various extracts of C. tora shows that the maximum number of chemical constituents present in methanolic extract in comparison to ethanol, ethyl acetate, acetone, and aqueous extracts of C. tora in given solvent system of toluene, ethyl acetate and glacial acetate. Further bioactivity guided fractionation and analysis of isolated chemical entity can reveal the active constituents in the various extracts of C. tora. Phytochemical analysis revealed the presence of carbohydrates, proteins. glycosides, sapnonins, flavonoids, phenolics and tannins, phytosterols and triterpenoids.

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