Hands-on Workshop on Cryobiology and Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse

Authors

  • Shilpa Kumari BA Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Reena V Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA Author
  • Srinivasulu T Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA Author
  • Manjunath A M Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Mahima N Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA Author
  • Vaishak Nair Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Salil Hangekar Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Priya P Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Akash Aswini Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Roopa N Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Abhishek Anand Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Latha Chukki Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Mohan GH Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author
  • Mahesh Sahare Mouse Genome Engineering Facility & Animal Care and Resource Center, National Centre for Biological Sciences (NCBS) and the Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore-560065, INDIA. Author

DOI:

https://doi.org/10.48165/jlas.2024.7.1.5

Keywords:

3Rs, Cryopreservation, Cryorepository, ARTs, Sperm and Embryo cryopreservation, Hands-on workshop, skill India

Abstract

Animal models are important in biomedical research for validation of in vitro results, conducting proof of concept studies,  and investigating the variety of disease processes and therapeutic targets that help in narrowing the gap between bench  and bedside. The advent of precise genome engineering technologies has allowed the generation of an increasing number  of novel and complex genetically engineered mouse models that can be made available to the scientific and research  community. It is important to also provide access to efficient technologies to safely archive, protect, manage, and maintain  these precious mouse models. Assisted Reproductive Technologies (ARTs) are emerging as an important tool in the  strategic management of vivariums, which involves animal welfare, disaster preparedness, cost minimization, and most  importantly, reproducible research. 

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References

Longenecker G, Cho K, Khillan JS, Kulkarni AB (2021). Cryopreservation Protocols for Genetically Engineered Mice. Curr Protoc. 1(5): e138.

Takeo T, Sztein J, Nakagata N (2019). The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm. Methods Mol Biol. 1874:243- 256.

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Published

2024-07-30

How to Cite

Hands-on Workshop on Cryobiology and Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse . (2024). Journal of Laboratory Animal Science, 7(1), 31-34. https://doi.org/10.48165/jlas.2024.7.1.5