Hands-on Workshop on Cryobiology and Assisted Reproductive Technologies (ARTs) in the Laboratory Mouse
DOI:
https://doi.org/10.48165/jlas.2024.7.1.5Keywords:
3Rs, Cryopreservation, Cryorepository, ARTs, Sperm and Embryo cryopreservation, Hands-on workshop, skill IndiaAbstract
Animal models are important in biomedical research for validation of in vitro results, conducting proof of concept studies, and investigating the variety of disease processes and therapeutic targets that help in narrowing the gap between bench and bedside. The advent of precise genome engineering technologies has allowed the generation of an increasing number of novel and complex genetically engineered mouse models that can be made available to the scientific and research community. It is important to also provide access to efficient technologies to safely archive, protect, manage, and maintain these precious mouse models. Assisted Reproductive Technologies (ARTs) are emerging as an important tool in the strategic management of vivariums, which involves animal welfare, disaster preparedness, cost minimization, and most importantly, reproducible research.
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References
Longenecker G, Cho K, Khillan JS, Kulkarni AB (2021). Cryopreservation Protocols for Genetically Engineered Mice. Curr Protoc. 1(5): e138.
Takeo T, Sztein J, Nakagata N (2019). The CARD Method for Mouse Sperm Cryopreservation and In Vitro Fertilization Using Frozen-Thawed Sperm. Methods Mol Biol. 1874:243- 256.