Evaluation of certain bioactive compounds  using in ovo diabetic model

Satish    T P

doi:10.48165/jlas.2019.1.1.8

Authors

Satish T P Department of Toxicology, Sun Pharma Advanced Research Co. Ltd. Vadodara 390010, Gujarat, IndiaP, Department of Toxicology, Sun Pharma Advanced Research Co. Ltd. 907/4, GIDC – Makarpura, Vadodara – 390010, Gujarat Author

DOI:

https://doi.org/10.48165/jlas.2019.1.1.8

Keywords:

environmental, enrichment,, welfare,, stress,, animal behavior

Abstract

Mammalian models are frequently used for drug research and delivery systems. However, valid mammalian models are expensive, time consuming and not easy to set up and evaluate. Furthermore, they are often linked with ethical and legal aspects. Many micro, macro nutrients and other photochemical from food materials are found to possess many desirable health benefits. A large number of studies involving animals have been carried out to explore these beneficial properties. As an alternative, chick embryo developed through incubation of fertile chicken eggs has been used as a novel system to develop suitable models for biochemical and neutraceutical research. The in ovo technique is accepted as an alternative to traditional mammalian models and this can be suitably used in our routine biochemical tests, wherever the animal trials are not feasible. In view of the above, the development and evaluation of certain bioactive components using in ovo technique as a diabetic model was carried out in our laboratory. Bixin decreased significantly the plasma glucose, triglycerides, cholesterol, urea, creatinine and blood urea nitrogen, while liver glycogen levels in treated diabetic chick embryos increased when compared to control diabetic chick embryos.

Downloads

Download data is not yet available.

References

Batchelor, G. R. (1991). Group housing on floor pens and environmental enrichment in sandy lop rabbits (I). Animal Technology, 42, 109-120.

Beaver, B. V. (1989). Environmental enrichment for laboratory animals. ILAR News, 31(2), 5-11.

Brain, P. F. (1992). Understanding the behaviors of feral species may facilitate design of optimal living conditions for common laboratory rodents. Animal Technology, 43, 99-105.

Carder, B., & Berkowitz, K. (1970). Rats' preference for earned food in comparison with free food. Science, 169, 1273-1274.

Davys, J. S. (1994). The floor pens for laboratory animals: A mixed blessing? Animal Technology, 45, 95-100.

Galef, B. G. Jr. (1999). Environmental enrichment for laboratory rodents: Animal welfare and the methods of science. Journal of Applied Animal Welfare Science, 2(4), 267-280.

Gray, G. (1988). Guinea pigs. Humane Innovations and Alternatives in Animal Experimentation, 2, 48-49.

Gunn, D., & Morton, D. B. (1995). Inventory of the behavior of New Zealand White rabbits in laboratory cages. Applied Animal Behaviour Science, 45, 277-292.

Haemisch, A., & Gärtner, K. (1994). The cage design affects intermale aggression in small groups of male laboratory mice: Strain-specific consequences on social organization, and endocrine activations in two inbred strains (DBA/2J and DBA/J). Journal of Experimental Animal Science, 36, 101-116.

Hetts, S., Clark, J. D., Calpin, J. P., Arnold, C. E., & Mateo, J. M. (1992). Influence of housing conditions on beagle behavior. Applied Animal Behaviour Science, 34, 137-155.

Hubrecht, R. C. (1993). A comparison of social and environmental enrichment methods for laboratory-housed dogs. Applied Animal Behaviour Science, 37, 345-361.

Hutchinson, E., Avery, A., & Vandewoude, S. (2005). Environmental enrichment for laboratory rodents. ILAR Journal, 46(2), 148-161.

Love, J. A., & Hammond, K. (1991). Group-housing rabbits. Lab Animal, 20(8), 37-43.

Moraska, A., & Fleshner, M. (2001). Voluntary physical activity prevents stress-induced behavioral depression and anti-KLH antibody suppression. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 281, 484-489.

Mortell, N. (2001). Practical environmental enrichment for rats and mice: The results of a survey. Animal Technology, 52, 1-17.

Perez, C., Canal, J. R., Domimgues, E., Campillo, J. E., Guillen, M., & Torres, M. D. (1997). Individual housing influences certain biochemical parameters in the rat. Laboratory Animals, 31, 357-361.

Riley, V. (1981). Psychoneuroendocrine influences on immunocompetence and neoplasia. Science, 212, 1100-1109.

Schwartz, R., Sackler, A., & Weltman, A. (1974). Adrenal relationships and aggressiveness in isolated female mice. Experientia, 30, 199-200.

Sparling, J. E., Mahoney, M., Baker, S., & Bielajew, C. (2009). The effects of gestational and postpartum environmental enrichment on the mother rat: A preliminary investigation. Behavioural Brain Research, 208(1), 213-223.

Stauffacher, M. (1992). Group housing and enrichment cages for breeding, fattening, and laboratory rabbits. Animal Welfare, 1, 105-125.

Vanderlip, S. L., Vanderlip, J. E., & Myles, S. (1985). A socializing program for laboratory-raised canines. Part 2: The puppy socialization schedule. Lab Animal, 14(2), 27-32.