Foresight vision on exogenous applications of in vitro derived dsRNAs in Agriculture
DOI:
https://doi.org/10.5958/2582-2683.2021.00009.5Keywords:
RNAi, siRNA, dsRNA, gene silencing, environmental risksAbstract
RNA interference (RNAi) is a gene regulatory mechanism that limits the transcript level by either suppressing transcription (transcriptional gene silencing [TGS]) or by activating a sequence-specific RNA degradation process (posttranscriptional gene silencing [PTGS]). The discovery of RNA silencing via RNAi has facilitated major recent breakthroughs in medicine, veterinary, agriculture, and environmental sciences. As such, the use of RNAi in agricultural fields presents an environmental friendly approach to generate pest- and pathogen-resistant crops. Spray-induced gene silencing (SIGS) provides an intelligent method of using double-stranded (ds)RNA as a trigger to silence target genes in pests and pathogens without side-effects like in the case of chemical pesticide use. This review examines the risks associated with accumulation of dsRNA and small interfering RNA (siRNA) in plants and invasive targeted organisms and environmental contamination, transgeneratioal gene silencing, dsRNA fate and future implementation of SIGS.
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Abdellatef E., Will T, Koch A., Imani J., Vilcinskas A., and Kogel K.H. 2015. Silencing the expression of the salivary sheath protein causes transgenerational feeding suppression in the aphid, Sitobion avenae. Plant Biotechnology Journal, 13: 849– 857.
Baum, JA., Bogaert, T., Clinton, W., Heck, GR., Feldmann, P., Ilagan, O., Johnson, S., Plaetinck, G., Munyikwa, T., Pleau, M., Vaughn, T., and Roberts, J. 2007. Control of coleopteran insect pests through RNA interference. Nature Biotechnology, 25: 1322–1326.
Bhatia, V., Bhattacharya, R., Uniyal, P., Singh, R., and Niranjan, R. 2012. Host generated siRNAs attenuate expression of serine protease gene in Myzus persicae. PLoS One, 7: e46343.
Baulcombe, D. 2004. RNA silencing in plants. Nature, 431: 356– 363.
Belles, X. 2010. Beyond Drosophila: RNAi in vivo and functional genomics in insects. Annual Review of Entomology, 55: 111– 128.
Bolognesi R., Ramaseshadri P., Anderson J., Bachman P., Clinton W., Flannagan R., Ilagan O., Lawrence C., Levine S., Moar W., Mueller G., Tan J., Uffman J., Wiggins E., Heck G., Segers G . 2012. Characterizing the mechanism of action of double stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte). PLoS One, 7: e47534
Coleman, AD., Wouters, R.H.M., Mugford, S.T., and Hogenhout, S.A. 2015. Persistence and transgenerational effect of plant mediated RNAi in aphids. Journal of Experimental Botany, 66: 541–548.
Dubelman, S., Fischer, J., Zapata, F., Huizinga, K., Jiang, C., Uffman, J., Levine, S., and Carson, D. 2014. Environmental Fate of Double-Stranded RNA in Agricultural Soils. PLoS One 9: e93155.
Feinberg, E.H., and Hunter, C.P. 2003. Transport of dsRNA into cells by the transmembrane protein SID-1. Science, 301: 1545–1547.
Friedman, A., and Perrimon, N. 2006. A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signaling. Nature, 444: 230–234.
Frizzi, A., and Huang, S. 2010. Tapping RNA silencing pathways for plant biotechnology. Plant Biotechnology Journal, 8: 655– 677.
Huang, G., Allen, R., Davis, E.L., Baum, T.J., and Hussey, R.S. 2006. Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root knot nematode parasitism gene. Proceedings of the National Academy of Sciences, USA, 103: 14302–14306.
Hunter, W.B., Glick, E., Paldi, N., and Bextine, B.R. 2012. Advances in RNA interference: dsRNA treatment in trees and grapevines for insect pest suppression. South west. Entomology, 37: 85–87.
Huvenne, H., and Smagghe, G. 2010. Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: A review Journal of Insect Physiology, 56: 227–235.
Ivashuta, S.I., Zhang, Y., Wiggins, B.E., Ramaseshadri, P., Segers, G.C., Johnson, S., Meyer, S.E., Kerstetter, R.A., McNulty, B.C., Bolognesi, R., and Heck, G.R. 2015. Environmental RNAi in herbivorous insects, RNA 21: 840–850.
Koch, A., Kumar, N., Weber, L., Keller, H., Imani, J., and Kogel, K.H. 2013. Host-induced gene silencing of cytochrome P450 lanosterol C14á-demethylase–encoding genes confers strong resistance to Fusarium species. Proceedings of the National Academy of Sciences, USA, 110: 19324–19329.
Koch, A., Biedenkopf, D., Furch, A., Weber, L., Rossbach, O., Abdellatef, E., Linicus, L., Johannsmeier, J., Jelonek, L., Goesmann, A., Cardoza, V., McMillan, J., Mentzel, T., and Kogel, K.H. 2016. An RNAi-based control of Fusarium graminearum infections through spraying of long dsRNAs involves a plant passage and is controlled by the fungal silencing machinery. PLoS Pathogens, 12: e1005901.
Konakalla, N.C., Kaldis, A., Berbati, M., Masarapu, H. and Voloudakis, A.E. 2016. Exogenous application of double-
Eltayb Abdellatef
stranded RNA molecules from TMV p126 and CP genes confers resistance against TMV in tobacco. Planta, 244: 961– 969.
Nowara, D., Gay, A., Lacomme, C., Shaw, J., Ridout, C., Douchkov, D., Hensel, G., Kumlehn, J. and Schweizer, P. 2010. HIGS: host-induced gene silencing in the obligate biotrophic fungal pathogen Blumeria graminis. Plant Cell, 22: 3130–3141.
McEwan, D.L., Weisman, A.S. and Huntert, C.P. 2012. Uptake of extracellular double-Stranded RNA by SID-2. Molecular Cell, 47: 746–754.
San Miguel K. and Scott J.G. 2016. The next generation of insecticides: dsRNA is stable as a foliar-applied insecticide. Pest Management Science, 72: 801–809.
Simon S.A. and Meyers B.C. 2010. Small RNA-mediated epigenetic modifications in plants. Current Opinion in Plant Biology, 14: 148–155.
Sindhu, A.S., Maier, T.R., Mitchum, M.G., Hussey, R.S. and Davis, E.L. 2009. Effective and specific in planta RNAi in cyst nematodes: expression interference of four parasitism genes reduces parasitic success. Journal of Experimental Botany, 60: 315–324.
Yadav, B.C., Veluthambi, K. and Subramaniam, K. 2006. Host generated double stranded RNA induces RNAi in plant parasitic nematodes and protects the host from infection. Molecular and Biochemical Parasitology, 148: 219–222.
Yoo, B.C., Kragler, F., Varkonyi-Gasic, E., Haywood, V., Archer Evans, S., Lee, Y.M., Lough, T.J. and Lucas, W.J. 2004. A systemic small RNA signaling system in plants. Plant Cell, 16: 1979–2000.
Vaucheret, H. and Chupeau, Y. 2012. Ingested plant miRNAs regulate gene expression in animals. Cell Research 22: 3–5. Wang, M., Weiberg, A., Lin F-M., Thomma, B.P.H.J., Huang, H.- D., and Jin, H. 2016. Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection. Nature Plants, 2: 16151.
Wierzbicki, A.T., Haag, J.R. and Pikaard, C.S. 2008. Noncoding transcription by RNA polymerase Pol IVb/Pol V mediates transcriptional silencing of overlapping and adjacent genes. Cell 135:635–648.
Winston, W.M., Sutherlin, M., Wright, A.J., Feinberg, E.H., and Hunter, C.P. 2007. Caenorhabditis elegans SID-2 is required for environmental RNA interference. Proceedings of the National Academy of Sciences, USA 104: 10565–10570.
Witwer, K.W. and Hirschi, K.D. 2014. Transfer and functional consequences of dietary microRNAs invertebrates: concepts in search of corroboration. Bioessays, 36: 394–406.
Whangbo, J.S. and Hunter, C.P. 2008. Environmental RNA interference. Trends in Genetics, 24: 297–305.
Zhang, X., Henderson, I.R., Lu, C., Green, P.J. and Jacobsen, S.E. 2007. Role of RNA polymerase IV in plant small RNA metabolism. Proceedings of the National Academy of Sciences, USA 104: 4536–4541.
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