Evaluation of solid and liquid substrates for mass proliferation of Trichoderma spp.
DOI:
https://doi.org/10.5958/2582-2683.2023.00034.5Keywords:
Evaluation, sabstrates, mass production, Trichoderma sppAbstract
Laboratory evaluation of different substrates for their suitability in mass production of five isolates of Trichoderma spp. revealed that the potato dextrose broth media providing largest population in isolate 1 (277.7 ×104 cfu g-1), isolate 2 (196.3 ×104 cfu g-1), isolate 3 (247.7 ×104 cfu g-1), isolate 4 (266.3 ×104 cfu g-1), and isolate 5 (237.3×104 cfu g-1) after two months of storage proved the best. Different species’ populations dropped gradually with the pace of loss varying with substrate. The lowest population of all the five species of Trichoderma was produced with rice straw.
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Adekunel, A. T., K. F. Cardwell, D. A., Florini and Ilkotum, T. 2001. Seed treatment with Trichoderma species for control of damping-off of cowpea caused by Macrophomina phaseolina. Biocontlrol Science and Technology, 11: 449-457
Bennett, A., C. Leifert and J. Whipps. 2006. Effect of combined treatment of pasteurization and Coniothyrium on sclerotia of Sclerotina sclerotiorum in soil. European Journal of Plant Pathology, 113: 197-209.
Chaudhari P J, Shrivastava P and Khadse AC. 2010. Substrate evaluation for mass cultivation of Trichoderma viride. Asiatic Journal of Biotechnology Research, 2: 441-446.
Coban, I and Sargin, S. 2019. Production of Trichoderma micropropagules as a biocontrol agent in static liquid culture conditions by using an integrated bioreactor system. Journal of Biocontrol Science and Technology, 29: 1197-1214.
Cook, R. J. and Baker, K. F. 1983. The nature and practice of biological control of plant pathogens. APS Press, St. Paul, USA. 539pp.
Harman, G. E. and Kubicek, C. P. 1998. Trichoderma and Gliocladium, Vol. 1 and 2 Taylor and Francis, Mycoparasitism of Gliocladium virens on London. p. 278.
Jeyarajan, R. 2006. Prospects of indigenous mass production and formulation of Trichoderma. In: Rabindra, R. J. and Ramanujam, B. (Eds.), Current Status of Biological Control
of Plant Diseases Using Antagonistic Organisms in India. Project Directorate of Biological Control, Bangalore. 445 pp.
Kaushal S and Chandel S. 2017. Enhancing the shelf life of Trichoderma species by adding antioxidants producing crops to various substrates. Journal of Crop Protection, 6: 307- 314.
Khandelwal, M., Datta, S., Mehta, J., Naruka, R., Makhijani, K., Sharma, G., Kumar, R. and Chandra, S. 2012. Isolation, characterization and biomass production of Trichoderma viride using various agro products- A biocontrol agent. Advance in Applied Science Research, 3: 3950-3955.
Lewis, J.A. and G.C. Papavizas. 1984. Chlamydospores formation by Trichoderma spp. In natural substrates. Canadian Journal of Microbiology, 30: 1-7.
Mamo Z and Alemu T. 2012. Evaluation and optimization of agro- industrial wastes for conidial production of Trichoderma isolates under solid state fermentation. Journal of Applied Bioscience, 54: 3870-3879.
Pan S and Das A. 2010. Evaluation of shelf life of some value added organic formulations of Trichoderma harzianum. The Journal of Plant Protection Science, 2: 33-37.
Rini, C.R. and K.K. Sulochana. 2007. Substrate evaluation for multiplication of Trichoderma spp. Journal of Tropical Agriculture, 45: 58-60.
Saju, K. A., Anandaraj, M. and Sharma,Y. R. 2002. On farm production of Trichoderma harzianum using organic matter. Indian Phytopathology, 55: 277-281.
Sargin S, Gezgin Y Eltem R and Vardar F. 2013. Micropropagule production from Trichoderma harzianum using solid state fermentation and study for comparative drying method. Turkish Journal of Biology, 101-112.
Sathiyaseelan, K., Sivasakthivelan, P. and Lenin, G. 2009. Evaluation of Antagonastic Activity and shelf life study of Trichoderma viride. Botany Research International, 2: 195-197.
Spiers T P Almer, P. Wood, T. Reglinski and K. Tate. 2005. Multiple strategies for effective pathogen control. New Zealand Plant Protection, 58: 62-67.
Subash N, Viji J, Sasikumar C and Meenakshisundaram M. 2013. Isolation, media optimization and formulation of Trichoderma harizanum in agricultural soil. Journal of Microbiology Biotechnology Research, 3: 61-64.
Tewari L and Bhanu C. 2004. Evaluation of agro-industrial wastes for conidia bases inoculum production of bio-control agent Trichoderma harzianum. Journal of Science and Industrial Research, 6: 807-812.
Zaidi, N.W. and U.S. Singh. 2004. Use of farm yard manure for mass multiplication and delivery of biocontrol agents, Trichoderma harzianum and Pseudomonas fluorescens. Asian Agriculture History, 52: 165-172.
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