Effect of different solid media and liquid media on growth, sporulation and biomass of Hirsutella thompsonii Fisher
DOI:
https://doi.org/10.48165/jefa.2023.18.02.18Keywords:
Biomass, growth, Hirsutella thompsonii, liquid media, solid media, sporulationAbstract
The experiment on effect of different solid and liquid media on growth, sporulation and biomass of H. thompsonii was conducted at Bio-control research laboratory, Department of Entomology, Navsari Agricultural University during the year of 2020-21. The result revealed that among the five solid media sabouraud’s dextrose agar+yeast medium supported higher radial growth, sporulation and biomass (wet and dry) of H. thompsonii followed by PDA medium. Five liquid media tested for sporulation and biomass of H. thompsonii, significantly highest sporulation, as well as wet and dry mycelial biomass, was observed in sabaurad dextrose broth, whereas significantly lowest spore production and biomass (mycelial wet and dry weight) was recorded in czapek’s dox dextrose broth.
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Agale, S.V., Gopalakrishnan, S., Ambhure, K.G., Chandravanshi, H., Gupta, R. and Wani, S.P. 2018. Mass production of entomopathogenic fungi (Metarhizium anisopliae) using different grains as a substrate. International Journal of Current Microbiology and Applied Sciences, 7 : 2227-2232.
Aghajanzadeh, S., Mallik, B. and Chandrashekar, S.C. 2006. Mass culturing of Hirsutella thompsonii Fisher a fungal pathogen of eriophyid mites. Pakistan Journal of Biological Sciences, 9 : 876-879.
Banik, S. and Halder, J. 2013. Acaropathogenic and entomopathogenic fungus Hirsutella- A review. Annals of Entomology, 31 : 143-155.
Banu, G.J. and Rajalakshmi, S. 2014. Standardization of media for mass multiplication of entomopathogenic fungi. International Journal of Plant Protection, 42 : 91-93.
Bhadauria, B.P., Puri, S. and Singh, P.K. 2012. Mass production of entomopathogenic fungi using agricultural products. The Bioscan, 7 : 229-232.
Carolino, A.T., Teodoro, T.B.P., Gomes, S.A., Silva, C.P. and Samuel, R.I. 2021. Production of conidia using different culture media modifies the virulence of the entomopathogenic fungus Metarhizium against Aedes aegypti larvae. Journal of Vector-Borne diseases, 58 : 346-351.
Dale, N.S. and Shinde, S.S. 2017. Growth, sporulation and biomass production of native entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin on a suitable medium. International Journal of Entomological Research, 2 : 93-98.
Deb, L., Thangaswamy, R. and Hajong, M. 2017. Growth of Beauveria bassiana in different solid media. Trends in Bioscience, 10 : 4815-4817.
Hajek, A.E. and Leger, R.J. 1994. Interactions between fungal pathogens and insects’ hosts. Annual review of Entomology, 39 : 293-322.
Hardin, M.R., Benrey, B., Coli, M., Lamp, W.O., Roderick, G.K., and Barbosa, P. 1995. Arthropod pest resurgence: An overview of potential mechanisms. Crop Protection, 14 : 3- 18.
Inobeme, A., Mathew, J.T., Okonkwo, S., Ajai, A.I., Jacob, J.O. and Olori, E. 2020. Pesticide residues in food: distribution, route of exposure and toxicity: in review. MOJ Food Processing and Technology, 8 : 121-124.
Kumar, D., Singh, K.P. and Jaiswal, R.K. 2005. Screening of different media and substrates for cultural variability and mass culture of Arthrobotrys dactyloides Drechsler. Mycobiology, 33 : 215-222.
Kumar, M., Prasanna, R., Bidyarani, N., Babu, S., Mishra, B.K., Kumar, A., Adak, A., Jauhari, S., Yadav, K. and Singh, R. 2013. Evaluating the plant growth-promoting ability of thermotolerant bacteria and cyanobacteria and their interactions with seed spice crops. Scientia Horticulturae, 164 : 94-101.
Lal, S., Rajak, D.C., Sinha, O.K. and Singh, V. 2010. Effect of different media on growth and sporulation of Metarhizium anisopliae, a fungal bioagent. Journal of Biological Control, 24 : 80-81.
McCoy, C.W. 1981. Fungi. Pest control by Hirsutella thompsonii. In: Microbial Control of Insects, Mites and Plant Diseases. (Ed. Burges, E.D.) Academic Press, London, UK, pp. 499-512.
McCoy, C.W., Samson, R.A. and Boucias, D.G. 1988. Entomogenous Fungi. In: Handbook of Natural Pesticides, Vol. V. Microbial Insecticides, Part A. (Eds. Ignoffo, C.M. and Mandava, N.B.). CRC Press, Boca Raton, pp. 151-236.
Pandey, R. and Kanaujia, K.R. 2010. Suitability of different synthetic and grain-based liquid media for the mass culture of entomopathogenic fungi, Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin. Journal of Entomological Research, 34 : 305-309.
Patil, S.D. 2015. Influence of different media on growth, biomass production, sporulation and concentrations of liquid form of Nomuraea rileyi inoculum’s on its growth, development and bioefficacy against Spodoptera litura. International Journal of Plant Protection, 8 : 288-294.
Prabowo, H., Yulianti, T., Damaiyani, J. and Fudholi, A. 2020. Effect of temperature and medium composition on growth of Metarhizium anisopliae, entomopathogenic fungi of sugarcane white grubs. International Journal of Advanced Science and Technology, 29 : 4490-4499.
Prasad, C.S. and Pal, R. 2014. Mass production and economics of entomopathogenic fungus, Beauveria bassiana, Metarhizium anisopliae and Verticillium lecanii on agricultural and industrial waste. Scholars Journal of Agriculture and Veterinary Sciences, 1 : 28-32.
Soundarapandian, P. and Chandra, R. 2007. Mass production of endomopathogenic fungus Metarhizium anisopliae (Deuteromycota, Hyphomycetes) in the laboratory. Research Journal of Microbiology, 2 : 690-695.
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