Cultural and Morphological Variability of Botrytis sp. isolates Causing Grey Mould of Gladiolus in India
DOI:
https://doi.org/10.48165/jefa.2023.18.02.31Keywords:
Botrytis cinerea, BGM, Gladiolus, PCMAbstract
The gladiolus is one of the important cut flower with the utmost commercial value in India. Gladiolus has about 180 species and over 30,000 cultivars, of which over 20 are produced in India for production of flowers commercially. Botrytis grey mould (BGM) caused by the fungus Botrytis cinerea is one of the most destructive disease of the gladiolus. In a present study, a total 35 isolates of Botrytis were obtained from the gladiolus surveyed area. Colony morphological studies (texture, form, color, production and degree of sporulation) has been conducted using three types of solid media i.e. PDA (Potato Dextrose Agar), Czapeck’sDox Agar, Kritzmen and Netzer Agar medium. All B. cinerea strains gave maximum fungal mycelium growth on PDA (90.0 mm to 87.5mm) and CDA than K and N medium. Spores of Botrytis cinerea was also observed under Scanning Electron Microscope (SEM) and Phase Contrast Microscope (PCM) for conidial size and number of conidia. Maximum conidial size was recorded on PDA in comparison to CDA and KN media.
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Cultural and morphological variability of Botrytis sp. isolates causing grey mould of Gladiolus in India Hosen, M.I. Ahmed, A.U. and Islam, M.R. 2010. Physiological
Ahmad, Q. 2007. Field screening of chickpea varieties and cultures against grey mold (Botrytis cinerea) in Bihar, India. International Chickpea Newsletter, 21 : 23.
Chardonnet, C., Sams, C. and Trigiano, R. 2000. Variability of three isolates of Botrytis cinerea affects the inhibitory effects of calcium on this fungus. Phytopathology, 90 : 769-774.
Dimock, A.W. 1940. Epiphytotic of Botrytis blight on gladiolus in Florida. Plant Disease Research, 24 : 159-161.
Elad, Y., Williamson, B., Tudzynski, P. and Delen, N. 2007. Botrytis spp. and diseases they cause in agricultural systems - an introduction. In: Botrytis: Biology, Pathology and Control, Springer Science & Business Media, The Netherlands. pp. 1-8.
Ellis, M.B. 1971. Dematiaceous Hypomycetes. Commonwealth Mycological Institute. Surrey. U.K. p. 1-608.
Fekete, E., Irinyi, L., Karaffa, L., Árnyasi, M. and Asadollahi, M. 2012. Genetic diversity of a Botrytis cinerea cryptic species complex in Hungary. Microbiology Research, 167 : 283-291.
Fernández, J.G., Fernández, Baldo, M.A., Sansone, G., Calvente, V., Benuzzi1, D., Salinas, E. and Raba, J. 2014. Effect of temperature on the morphological characteristics of Botrytis cinerea and its correlation with the genetic variability. Journal of Coastal Life Medicine, 2 : 543-548.
Fournier, E., Giraud, T., Alberini, C. and Brygoo, Y. 2005. Partition of the Botrytis cinerea complex in France using multiple gene genealogies. Mycologia, 97 : 1251-67.
Hala Abdel, W., Elsayed, E.W., Mohamed, R.A.S., Magda, M.F. and Mahmoud, A.G. 2020 Studies on Botrytis spp. infected ornamental plants and emergence of resistant isolates against Fenhexamid in Egypt. Asian Journal of Plant Pathology, 14 : 1-10.
Hennebert, G.L. 1973. Botrytis and Botrytis like genera. Persoonia, 7 : 193-204.
Hosen, M.I. 2011. Cultural, physiological comparison and fungicidal sensitivity between two isolates of Botrytis cinerea and Stemphylium botryosum. Emirates Journal of Food and Agriculture, 23 : 120-129.
variability and in vitro antifungal activity against Botrytis cinerea causing botrytis gray mold of chickpea (Cicer arietinum L.). Spanish Journal of Agricultural Research, 8 : 750- 856.
Jarvis, W. 1977. Botryotinia and Botrytis species- Taxonomy and Pathogenicity. Canada Department of Agriculture, Monograph, 15, Harrow, Ontario, Canada.
Kaur, G. and Chandel, S. 2016. First report of Botrytis cinerea causing grey mould of gladiolus in India. Journal of Plant Pathology, 98 : 171-185.
Khazaeli, P., Zamanizadeh, H., Morid, B. and Bayat, H. 2010. Morphological and molecular identiûcation of Botrytis cinerea causal agent of gray mold in rose greenhouses in central regions of Iran. International Journal of Agricultural Science and Research, 1 : 19-24.
Martinez, F., Coriocostet, M. F., Levis, C., Coarer, M. and Fermaud, M. 2008. New PCR primers applied to characterize distribution of Botrytis cinerea population in French vineyards. Vitis, 47 : 217-226.
Mirzaei, S., Goltapeh, E.M., Shamsbakhsh, M., Safaie, N. and Chaichi, M. 2009. Genetic and phenotypic diversity among Botrytis cinerea isolates in Iran. Journal of Phytopathology, 157: 474-482.
Tanovic, B., Delibasicg, Milivojevic, J. and Nikolic, M. 2009. Characterization of Botrytis cinerea isolates from the small fruits and grapevine in Serbia. Archives of Biological Sciences, 61 : 419-429.
Vercesi, A., Toffolatti, S.L., Venturini, G., Campia, P. and Scagnelli, S., 2014. Characterization of Botrytis cinerea population associated with treated and untreated cv. Moscato vineyards. Phytopathologia Mediterranea, 5 : 108-123.
Xie, X.W., Zhang, Z.X., Chai, A.L., Shi, Y.X. and Li, B.J. 2016. Grey mould on leaf mustard caused by Botrytis cinerea, a new disease in China. Australasian Plant Diseases Notes, 11 : 1-4.
Zhou, Y.J., Zhang, J. and Wang, H.D. 2014. Morphological and phylogenetic identification of Botrytis sinoviticola, a novel cryptic species causing gray mold disea106 : 43-56.
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