The Identification of single nucleotide polymorphisms in GnRH-I, GnRH-II/MRPS26, GnRHR exons and their Association with egg production in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken

Authors

  • Sourav Pal Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat - 388001, India. https://orcid.org/0000-0002-5349-8610
  • Ashish C. Patel Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat - 388001, India. https://orcid.org/0000-0002-2770-0440
  • Ankit T. Hinsu Project Scientist-III, Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat – 388001. https://orcid.org/0000-0002-8985-4541
  • Ketankumar J. Panchal Research Associate, Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat – 388001. https://orcid.org/0000-0002-6571-5889
  • Prakash G. Koringa Assistant Professor, Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat – 388001. https://orcid.org/0000-0002-7709-4231
  • Dharamsibhai N. Rank Professor, Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Anand, Gujarat – 388001. https://orcid.org/0000-0002-7321-0127

DOI:

https://doi.org/10.48165/ijvsbt.19.2.18

Keywords:

SNP, chicken, egg production, GnRH, amplicon sequencing, white leghorn

Abstract

The aim of study was to identify Single Nucleotide Polymorphisms (SNPs) in the exon regions of GnRH-I, GnRH-II/MRPS26 and GnRHR genes and to assess their association with egg production up to 64 weeks of age (EN64) in chicken. Blood samples were collected from 48 Anand Synthetic White Leghorn (ASWLH) and 48 Anand Bantamised White Leghorn (ABWLH) chicken. Custom panel was designed for these genes’ exon regions and amplicon sequencing was performed on Illumina Miseq platform. Total 41 SNPs (19 previously reported and 22 novel SNPs) were identified from 96 samples. Association analysis revealed that rs314996211 (p<0.05), rs313100310 (p<0.01), g.17308550T>C (p<0.01) of GnRHR were significantly associated with EN64. Conclusively, rs314996211, rs313100310, g.17308550T>C of GnRHR can be implemented as selection markers associated with EN64 in chicken.

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Published

2023-03-02

Issue

Vol. 19 No. 2 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Mar- Apr) 2023

Section

Articles

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Copyright (c) 2023 Indian Journal of Veterinary Sciences & Biotechnology

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How to Cite

Pal, S., Patel, A.C., Hinsu, A.T., Panchal, K.J., Koringa, P.G., & Rank, D.N. (2023). The Identification of single nucleotide polymorphisms in GnRH-I, GnRH-II/MRPS26, GnRHR exons and their Association with egg production in Anand Synthetic White Leghorn and Anand Bantamised White Leghorn Chicken. Indian Journal of Veterinary Sciences and Biotechnology, 19(2), 94–98. https://doi.org/10.48165/ijvsbt.19.2.18
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