Pathological and Molecular Study of Infectious Bursal Disease in Commercial Chicken
DOI:
https://doi.org/10.48165/ijvsbt.19.1.08Keywords:
Gross lesions, Histopathology, Infectious bursal disease, Poultry, DiagnosisAbstract
A total of 100 Poultry carcasses suspected for infectious bursal disease were collected from 20 poultry farms and conducted postmortem at the Department of Veterinary Pathology, NTR College of Veterinary science, Gannavaram ( Andra Pradesh , India) . Sick birds showed ruffled feathers, loss of appetite, whitish diarrhea and soiled vent. On postmortem examination, grossly bursa of Fabricius was swollen, enlarged with mucous exudate in lumen and haemorrhages were noticed on the mucosa. Paint brush haemorrhages were noticed on thigh muscles and at the junction of gizzard and proventriculus. Kidneys were swollen and ureters were filled with urate crystals. Histopathology of bursa of Fabricius revealed shrinkage of follicles, severe lymphoid depletion, necrosis, lymphocytolysis and cyst formation in follicles. Diagnosis of the disease was confirmed by targeting the VP2 gene of infectious bursal disease virus (IBDV) with a reverse transcriptase polymerase chain reaction which revealed an amplicon size of 743bp. Based on gross and histopathological lesions and molecular findings disease was confirmed as infectious bursal disease.
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Agnihotri, A. A., Awandkar, S. P., Kulkarni, M. B., Chavhan, S. G., Kulkarni, R. C., &Chavan, V. G. (2022). Molecular phylodynamics of infectious bursal disease viruses. Virus Genes, 1-11.
Akram, T., Muneeb, J. M., Gul, I., Parveez, M., Shah, R. A., Ahmad, S. M.,& Shabir, N. (2020). Isolation of infectious bursal disease virus from a poultry farm in Srinagar district of Jammu and Kashmir. SKUAST Journal of Research, 22(2), 86-91.
Deorao, C. V., Rajasekhar, R., Ravishankar, C., Nandhakumar, D., Sumod, K., Palekkodan, H., & Chaithra, G. (2021). Genetic variability in VP1 gene of infectious bursal disease virus from the field outbreaks of Kerala, India. Tropical Animal Health and Production, 53(3), 1-9.
Dey, S., Pathak, D. C., Ramamurthy, N., Maity, H. K., & Chellappa, M. M. (2019). Infectious bursal disease virus in chickens: prevalence, impact, and management strategies. Veterinary Medicine: Research and Reports, 10, 85.
Eterradossi, N. & Saif, Y. M. (2020) Infectious bursal disease. In Diseases of poultry. 14th edition, Ed: Swayne, D. E. et al., John Wiley & Sons, Inc., Hoboken, USA, Pp. 257-283.
Fahey, K.J., Erny, K., and Crooks, J. (1989). A conformational immunogen on VP2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens. Journal of General Virology , 70,1473–1481.
Fan, L., Wang, Y., Jiang, N., Gao, L., Li, K., Gao, Y., & Qi, X. (2020). A reassortment vaccine candidate of the novel variant infectious bursal disease virus. Veterinary Microbiology, 251, 108905.
Hou, B., Wang, C. Y., Luo, Z. B., & Shao, G. Q. (2022). Commercial vaccines used in China do not protect against a novel infectious bursal disease virus variant isolated in Fujian. Veterinary Record, e1840.
Jackwood, D. H., & Saif, Y. M. (1987). Antigenic diversity of infectious bursal disease viruses. Avian diseases, 766-770.
Jackwood, D. J., & Sommer-Wagner, S. E. (2005). Molecular epidemiology of infectious bursal disease viruses: distribution and genetic analysis of newly emerging viruses in the United States. Avian diseases, 49(2), 220-226.
Jackwood, D. J., Sreedevi, B., LeFever, L. J., & Sommer-Wagner, S. E. (2008). Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity. Virology, 377(1), 110-116.
Müller, H., & Nitschke, R. (1987). The two segments of the infectious bursal disease virus genome are circularized by a 90,000-Da protein. Virology, 159(1), 174-177.
Nandhakumar, D., Rajasekhar, R., Logeshwaran, G., Ravishankar, C., Sebastian, S. R., Anoopraj, R., & John, K. (2020). Identification and genetic analysis of infectious bursal disease viruses from field outbreaks in Kerala, India. Tropical animal health and production, 52(3), 989-997.
Omer, M. G., & Khalafalla, A. I. (2022). Epidemiology and laboratory diagnosis of very virulent infectious bursal disease virus in vaccinated chickens in Khartoum, Sudan. Open Veterinary Journal, 12(1), 33-43.
Pandey, M. K., Agrawal, D. K., Mishra, G. K., Gupta, V., & Raghuvanshi, P. D. S. (2021). Comparative haemato-biochemical and histopathological studies in birds inoculated with vaccine and field strain of infectious bursal disease virus. Indian Journal of Animal Research, 55(3), 315-323.
Prabhu, S. N., Singh, A. P., Varghese, B. P., Dhama, K., Singh, S. D., & Singh, R. (2020). A comparative study of pathology and host immune response induced by very virulent infectious bursal disease virus in experimentally infected chickens of Aseel and white Leghorn breeds. Vaccines, 8(4), 627.
Raja, P., Senthilkumar, T. M. A., Priyadarshini, C. V., Parthiban, M., Thangavelu, A., Gowri, A. M., & Kumanan, K. (2018). Sequence analysis of VP2 hypervariable region of the field isolates of infectious bursal disease viruses from southern region of India. Acta virologica, 62(1), 86-97.
Shinde, R. S., Chauhan, H. C., Patel, A. C., Sharma, K. K., Patel, S. S., Mohapatra, S. K. & Chandel, B. S. (2021). VP2 gene sequencing-based Geno-grouping of infectious bursal disease viruses isolated from Gujarat and Maharashtra state (India). VirusDisease, 32(4), 823-829.
Spackman, E., Stephens, C. B., & Pantin-Jackwood, M. J. (2018). The effect of infectious bursal disease virus–induced immunosuppression on vaccination against highly pathogenic avian influenza virus. Avian diseases, 62(1), 36-44.
Thai, T. N., Yoo, D. S., Jang, I., Kwon, Y. K., & Kim, H. R. (2022). Dynamics of the Emerging Genogroup of Infectious Bursal Disease Virus Infection in Broiler Farms in South Korea: A Nationwide Study. Viruses, 14(8), 1604.
Withers, D. R., Young, J. R., & Davison, T. F. (2005). Infectious bursal disease virus-induced immunosuppression in the chick is associated with the presence of undifferentiated follicles in the recovering bursa. Viral immunology, 18(1), 127-137.
Wu, T., Wang, Y., Li, H., Fan, L., Jiang, N., Gao, L., & Qi, X. (2020). Naturally occurring homologous recombination between novel variant infectious bursal disease virus and intermediate vaccine strain. Veterinary microbiology, 245, 108700.
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