Detection of Biofilm Forming Streptococcus species from Bovine Mastitis

Authors

  • D. K Parasana Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, 362001, Gujarat, India
  • B. B Javia Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, 362001, Gujarat, India
  • D. B Barad Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, 362001, Gujarat, India
  • D. T Fefar Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, 362001, Gujarat, India
  • S. N Ghodasara Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Junagadh, 362001, Gujarat, India

DOI:

https://doi.org/10.48165/ijvsbt.19.1.24

Keywords:

Biofilm, Bovine, Mastitis, Streptococcus

Abstract

Mastitis is a common disease condition of farm animals, which causes production loss in animals and heavy economic loss to the dairy farmers. Streptococcus genus is one of the important etiological agents for mastitis. Biofilm formation by organisms leads to chronic infection due to more antibiotic resistance. The purpose of our study was to determine the ability to produce biofilm among bacteria of the genus Streptococcus isolated from bovine mastitis. In present study, micro-titer plate assay was used to detect the biofilm formation in Streptococcus isolates. Out of 98 Streptococcus isolates, 30 (30.61%) were non-biofilm former, 43 (43.87%) were weak biofilm former, 15 (15.30%) were moderate biofilm former and 10 (10.20%) were strong biofilm former.

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References

Baselga R.A., Albizu I.N., De La Cruz M.O., Del Cacho E.M., Barberan M., & Amorena B. (1993). Phase variation of slime production in Staphylococcus aureus: implications in colonization and virulence. Infection and Immunity, 61(11), 4857-4862.

Ebrahimi, A., Moatamedi, A., Lotfalian, S., & Mirshokraei, P. (2013). Biofilm formation, hemolysin production and antimicrobial susceptibilities of Streptococcus agalactiae isolated from the mastitis milk of dairy cows in Shahrekord district, Iran. Veterinary Research Forum, 4(4), 269.

Felipe, V., Morgante, C.A., Somale, P.S., Varroni, F., Zingaretti, M.L., Bachetti, R.A., Correa, S.G., & Porporatto, C. (2017). Evaluation of the biofilm forming ability and its associated genes in Staphylococcus species isolates from bovine mastitis in Argentinean dairy farms. Microbial Pathogenesis, 104, 278-286.

Galie, S., García-Gutiérrez, C., Miguélez, E. M., Villar, C. J., & Lombó, F. (2018). Biofilms in the food industry: health aspects and control methods. Frontiers in microbiology, 9, 898.

Jamal, M., Ahmad, W., Andleeb, S., Jalil, F., Imran, M., Nawaz, M.A., Hussain, T., Ali, M., Rafiq, M., & Kamil, M.A. (2018). Bacterial biofilm and associated infections. Journal of The Chinese Medical Association, 81(1), 7-11.

Kaczorek, E., Małaczewska, J., Wójcik, R., & Siwicki, A. K. (2017). Biofilm production and other virulence factors in Streptococcus spp. isolated from clinical cases of bovine mastitis in Poland. BMC Veterinary Research, 13(1), 1-7.

Pedersen, R. R., Krömker, V., Bjarnsholt, T., Dahl-Pedersen, K., Buhl, R., & Jorgensen, E. (2021). Biofilm research in bovine mastitis. Frontiers in Veterinary Science, 449.

Rato, M.G., Bexiga, R., Florindo, C., Cavaco, L.M., Vilela, C.L., & Santos-Sanches, I. (2013). Antimicrobial resistance and molecular epidemiology of Streptococci from bovine mastitis. Veterinary Microbiology, 161(3-4), 286-294.

Ruegg, P.L. (2017). A 100-year review: Mastitis detection, management, and prevention. Journal of Dairy Science, 100(12), 10381-10397.

Saginur, R., St. Denis, M., Ferris, W., Aaron, S. D.,Chan, F., Lee, C., & Ramotar, K. (2006). Multiple combination bactericidal testing of staphylococcal biofilms from implant-associated infections. Antimicrobial Agents and Chemotherapy, 50(1), 55-61.

Sharma, N., Rho, G.J., Hong, Y.H., Kang, T.Y., Lee, H.K., Hur, T.Y., & Jeong, D.K. (2012). Bovine mastitis: an Asian perspective. Asian Journal of Animal and Veterinary Advances, 7, 454-476.

Sohail, M.N., Rathnamma, D., Isloor, S., Veeregowda, B.M., & Sharada, R. (2019). Detection of biofilm formation ability of Streptococcus agalactiae isolated from bovine mastitis cases. International Journal of Farm Sciences, 9(1), 107-115.

Stepanovic, S., Vukovic, D., Hola, V., Bonaventura, G.D., Djukic, S., Cirkovic, I., & Ruzicka, F. (2007). Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci. Apmis, 115(8), 891-899.

Tendolkar, P. M., Baghdayan, A. S., Gilmore, M. S., & Shankar, N. (2004). Enterococcal surface protein, Esp, enhances biofilm formation by Enterococcus faecalis. Infection and Immunity, 72(10), 6032-6039.

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Published

2023-01-10

How to Cite

Parasana , D.K., Javia, B.B., Barad, D.B., Fefar, D.T., & Ghodasara, S.N. (2023). Detection of Biofilm Forming Streptococcus species from Bovine Mastitis. Indian Journal of Veterinary Sciences and Biotechnology, 19(1), 106–108. https://doi.org/10.48165/ijvsbt.19.1.24