Detection of Mycobacterium Species by Targeting esxA, esxB and espC genes in Cattle and Buffaloes

Authors

  • Sumeet Singh Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Deepti Narang Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Mudit Chandra Department of Veterinary Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Randhir Singh Centre for One Health, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India
  • Jaswinder Singh Department of Veterinary Animal Husbandry and Extension Education, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141004, India

DOI:

https://doi.org/10.48165/ijvsbt.19.1.25

Keywords:

Bovine tuberculosis, Mycobacterium tuberculosis complex, Polymerase Chain Reaction

Abstract

Bovine tuberculosis (bTB) is a chronic disease having a potential zoonotic threat and has a huge economic importance due to morbidity, mortality and production losses. bTB is endemic in India and has a worldwide prevalence, therefore there is a need for early diagnostic technique for the eradication of bTB globally. The objective of this study was to diagnose bTB by targeting esxA, esxB and espC genes in the blood of cattle and buffaloes. A total of 223 blood samples were randomly collected from cattle and buffaloes with a history of respiratory distress. The samples were subjected to conventional PCR targeting esxA, esxB and espC genes.  A total of 12 samples out of 223 blood samples were found positive for esxA, esxB and espC by conventional PCR. Blood sample can be used for molecular detection of Mycobacterium tuberculosis organisms by targeting esxA, esxB and espC genes.

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References

Brahma, D., Narang,D., Chandra,M., Filia,G.,Singh, A. & Singh, S.T. (2019). Diagnosis of Bovine Tuberculosis by Comparative Intradermal Tuberculin Test, Interferon Gamma Assay and esxB (CFP-10)PCR in Blood and Lymph Node Aspirates. Open Journal of Veterinary Medicine,9,55-65.

Cezar, R.D.S., Silva, N.C., Filho, A.F.B.B., Borges, J.D.M., Oliveira, P.R.F.D., Lúcio, E.C., Lima, M.A., Santana, V.L.D.A. & Junior, J.W.P. (2016). Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil. BMC Veterinary Research,12(31), 1-6.

Dikshit, M., Sharma, R.J., Adsool, A.D.&Chaphalkar, S.R. (2012).ESAT-6 and CFP-10 proteins of Mycobacterium tuberculosis in making diagnostic tool for TB. Journal of Biotechnology letters,3,28-30.

Figueiredo, E.E.S., Silvestre, F.G., Campos, W.N., Furlanetto, L.V., Medeiros, L., Lilenbaum, W., Fonseca, L.S., Silva, J.T. &Paschoalin, V.M.F. (2009). Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR. Brazilian Journal of Microbiology,41, 2.

Gomez-Laguna, J., Carrasco, L., Ramis, G., Quereda, J.J., Gomez, S. &Pallares, F.J. (2010).Use of real-time and classic polymerase chain reaction assays for the diagnosis of porcine tuberculosis in formalin-fixed, paraffin-embedded tissues. Journal of Veterinary Diagnostics and Investigation, 22, 123-127.

Hunt, D.M., Sweeney, N.P., Mori, L., Whalan, R.H., Comas, I., Norman, L., Cortes, T., Arnvig, K.B., Davis, E.O., Stapleton, M.R. & Green, J. (2012). Long-range transcriptional control of an operon necessary for virulence-critical ESX-1 secretion in Mycobacterium tuberculosis. Journal of Bacteriology, 194(9), 2307-2320.

Kaya, A., Icen, H., Tuzcu, N., Simsek, A., Yesilmen, S., Kochan, A., Yildirim, I. & Alan, A. (2015). Comparison of Tuberculin Skin Test, IFN-γ Assay, Real Time PCR and Lateral FlowRapid Test in Diagnosis of Field Outbreaks. Bovine Tuberculosis,21(5), 739-743.

Millington, K. A., Fortune, S. M., Low, J., Garces, A., Hingley-Wilson, S. M., and Wickremasinghe, M., et al. (2011). Rv3615c is a highly immunodominant RD1 (Region of Difference 1)-dependent secreted antigen specific for Mycobacterium tuberculosis infection. Proc. Natl. Acad. Sci. U.S.A. 108, 5730–5735.

Pinxteren, L.A.H.V., Ravn, P., Agger, E.M., Pollock, J. & Andersen, P. (2000). Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP-10. Clinical and Diagnostic Laboratory Immunology, 7, 155-160.

Rogerson, B.J., Jung, Y.J., LaCourse, R., Ryan, L., Enright, N. & North, R.J. (2006). Expression levels of Mycobacterium tuberculosis antigen encoding genes versus production levels of antigen specific T cells during stationary level lung infection in mice. Immunology, 118, 195-201.

Scordo, J. M., Knoell, D. L., & Torrelles, J. B. (2016). Alveolar Epithelial Cells in Mycobacterium tuberculosis Infection: Active Players or Innocent Bystanders?. Journal of innate immunity, 8(1), 3–14.

Serrano-Moreno, B.A., Romero, T.A., Arriaga, C., Torres, R.A., Pereira-Suarez, A.L., Garcia-Salazar, J.A. & Estrada-Chavez, C. (2008). High Frequency of Mycobacterium bovis DNA in Colostra from Tuberculous Cattle Detected by Nested PCR. Zoonoses Public Health, 55, 258-266.

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Published

2023-01-10

Issue

Vol. 19 No. 1 (2023): The Indian Journal of Veterinary Sciences and Biotechnology (IJVSBT) (Jan- Feb) 2023

Section

Short Communication

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Singh, S., Narang, D., Chandra, M., Singh, R., & Singh, J. (2023). Detection of Mycobacterium Species by Targeting esxA, esxB and espC genes in Cattle and Buffaloes. Indian Journal of Veterinary Sciences and Biotechnology, 19(1), 109–111. https://doi.org/10.48165/ijvsbt.19.1.25
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